The resetting of the circadian rhythm by Prostaglandin J2 is distinctly phase-dependent
The resetting of the circadian rhythm by Prostaglandin J2 is distinctly phase-dependent
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DOI：
10.1016/j.febslet.2008.12.035
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发表时间：
2009-01-22
期刊：
影响因子：
3.5
通讯作者：
Shigeyoshi, Yasufumi
中科院分区：
文献类型：
--
作者：
Koinuma, Satoshi;Yagita, Kazuhiro;Shigeyoshi, Yasufumi
昼夜节律可以通过多种物质来重置。前列腺素 J(2) (PGJ(2)) 就是这样一种物质，它可以重置成纤维细胞的昼夜节律。在我们目前的研究中，我们使用实时荧光素酶发光监测系统检查了 PGJ(2) 处理后的相位依赖性相移。在相位响应曲线中，与毛喉素的相同分析相比，我们观察到峰值时间有 12 小时的差异。量。 PGJ(2) 给药后时钟基因 mRNA 的阳离子还显示 Per1、Rev-erbA α 和 Dbp 水平迅速下降。因此，我们目前的研究结果表明，PGJ(2) 通过一种不同于毛喉素 (FK) 所使用的机制来重置外周生物钟。 (C) 2008 年欧洲生化学会联合会。由 Elsevier B.V 出版。保留所有权利。
The circadian rhythm can be reset by a variety of substances. Prostaglandin J(2) (PGJ(2)) is one such substance and resets the circadian rhythm in fibroblasts. In our current study, we examined the phase-dependent phase shift following PGJ(2) treatment using a real-time luciferase luminescence monitoring system. In the phase response curves, we observed 12 h differences in the times of peaks in comparison with the same analysis for forskolin. Quanti. cation of clock gene mRNAs following PGJ(2) administration additionally revealed a rapid decrease in the Per1, Rev-erbA alpha and Dbp levels. Our current findings thus suggest that PGJ(2) resets the peripheral circadian clock via a mechanism that is distinct from that used by forskolin (FK). (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.