The expression of ceruloplasmin, an angiogenic glycoprotein, by mouse embryonic fibroblasts.
The expression of ceruloplasmin, an angiogenic glycoprotein, by mouse embryonic fibroblasts.
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小鼠胚胎成纤维细胞表达铜蓝蛋白（一种血管生成糖蛋白）。
DOI：
10.1016/0006-291x(85)90565-0
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发表时间：
1985
影响因子：
3.1
通讯作者：
Olden,K
中科院分区：
文献类型：
--
作者：
Chu,FF;Olden,K
摘要 Balb c 3T3、Swiss 3T3 和 Rous 肉瘤病毒转化的 Balb c 3T3 小鼠胚胎成纤维细胞在体外产生铜蓝蛋白，而从 Balb c 小鼠胚胎制备的原代培养物不产生铜蓝蛋白。 Balb c 3T3 细胞系的铜蓝蛋白合成量通过劳斯肉瘤病毒转化（1.5-3 倍）和地塞米松处理（约 2.4 倍）而增强。通过 (1) 使用铜蓝蛋白特异性多克隆抗体进行免疫沉淀，并通过 (ii) 肽图和 (iii) 亚基分子量（135,000 道尔顿）与来自小鼠肝细胞原代培养物的真实铜蓝蛋白的相似性，将该蛋白质鉴定为铜蓝蛋白。
Abstract Balb c 3T3, Swiss 3T3 and Rous sarcoma virus transformed Balb c 3T3 mouse embryonic fibroblasts produced ceruloplasmin in vitro, whereas primary cultures prepared from the Balb c mouse embryos did not produce ceruloplasmin. The amount of ceruooplasmin synthesis by the Balb c 3T3 cell line is enhanced by Rous sarcoma virus-transformation (1.5–3 fold) and by treatment with dexamethasone (about 2.4 fold). The protein was identified as ceruloplasmin by (1) immunoprecipitation with ceruloplasmin-specific polyclonal antibody, and by similarity of (ii) peptide maps, and (iii) subunit molecular weight (135,000 dalton) to that of authentic ceruloplasmin from primary cultures of mouse hepatocytes.