BRCA1 Accelerates CtIP-Mediated DNA-End Resection
BRCA1 Accelerates CtIP-Mediated DNA-End Resection
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DOI：
10.1016/j.celrep.2014.08.076
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发表时间：
2014-10-23
期刊：
影响因子：
8.8
通讯作者：
Huertas, Pablo
中科院分区：
文献类型：
--
作者：
Cruz-Garcia, Andres;Lopez-Saavedra, Ana;Huertas, Pablo
DNA末端切除是DNA双链断裂反应和修复过程中高度调控的关键步骤。在高等真核生物中，CtIP通过其翻译后修饰和蛋白质-蛋白质相互作用（包括与BRCA 1的细胞周期控制的相互作用）整合细胞信号来调节切除。BRCA 1在DNA末端切除中的作用尚不清楚。在这里，我们开发了一种检测方法来研究高分辨率的高等真核生物中的DNA切除。我们证明，BRCA 1-CtIP的相互作用，虽然不是必要的切除，调制的速度，这一过程发生。
DNA-end resection is a highly regulated and critical step in the response and repair of DNA double-strand breaks. In higher eukaryotes, CtIP regulates resection by integrating cellular signals via its posttranslational modifications and protein-protein interactions, including cell-cycle-controlled interaction with BRCA1. The role of BRCA1 in DNA-end resection is not clear. Here, we develop an assay to study DNA resection in higher eukaryotes at high resolution. We demonstrate that the BRCA1-CtIP interaction, albeit not essential for resection, modulates the speed at which this process takes place.