Characterization of inter-Sertoli cell tight and gap junctions in the testis of turtle: Protect the developing germ cells from an immune response
Characterization of inter-Sertoli cell tight and gap junctions in the testis of turtle: Protect the developing germ cells from an immune response
复制标题
乌龟睾丸中支持细胞间紧密连接和间隙连接的表征：保护发育中的生殖细胞免受免疫反应
DOI：
10.1016/j.micpath.2018.06.037
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发表时间：
2018-10-01
影响因子：
3.8
通讯作者：
Chen, Qiusheng
中科院分区：
文献类型：
--
作者：
Ahmed, Nisar;Yang, Ping;Chen, Qiusheng
可以想象的是，早期发育的生殖细胞必须跨整个基底到精子发生过程中的生精管（STS）的腔区域与连接络合物的广泛重组有关。但是，记录了有关爬行动物中这些连接综合体的非常有限的信息。在本研究中，我们确定了睾丸中精子发生期间的塞堡间细胞右连接（TJ），蛋白质CLDN11和GAP连接蛋白CX43的定位。在早期的精子发生中，在腔区域的Sertoli细胞周围观察到CLDN11和局灶性局灶性定位的弱免疫反应性，但与ST的基础隔室完全分层。在晚期精子发生中，在两个Sertoli细胞之间的接触点以及STS基底隔室中原代精子细胞的早期阶段和在原代精子细胞的早期阶段周围检测到了CLDN11和CX43的线性定位。在晚期精子发生中，Cldn11和Cx43的定位大幅度降低，仅在基底层附近的Sertoli细胞和精子周围看到。然而，透射电子显微镜表明，在精子发生中，在STS的基底室内存在近酚间细胞紧密连接，使精子症和早期原代精子细胞在基底区域中留下。在整个精子发生中，在Sertoli细胞和具有精子症和原代精子细胞的Sertoli细胞之间观察到了间隙连接。此外，在精子发生过程中观察到了粘附剂和半体体连接。上面的发现统称，TJ和间隙连接的强度和定位因精子发生阶段而有所不同，这些阶段可能会保护发育中的生殖细胞免受自身免疫反应的影响。
It is conceivable that early developing germ cells must across the basal to the luminal region of seminiferous tubules (STs) during spermatogenesis is associated with extensive restructuring of junctional complex. However, very limited information is documented about these junctional complexes in reptiles. In the present study we have determined the localization of inter-Sertoli cell right junctions (TJ's), protein CLDN11 and gap junction protein Cx43 during spermatogenesis in the testis. In early spermatogenesis, weak immunoreactivity of CLDN11and focal localization of Cx43 was observed around the Sertoli cell in the luminal region, but completely delaminated from the basal compartment of STs. In late spermatogenesis, strong focal to linear localization of CLDN11and Cx43 was detected at the points of contact between two Sertoli cells and around the early stages of primary spermatocytes in the basal compartment of STs. In late spermatogenesis, localization of CLDN11and Cx43 was drastically reduced and seen only around Sertoli cells and spermatogonia near the basal lamina. However, transmission electron microscopy revealed that inter-Sertoli cell tight junctions were present within the basal compartment of STs, leaving the spermatogonia and early primary spermatocytes in the basal region during mid spermatogenesis. Gap junctions were observed between Sertoli cells, and Sertoli cells with spermatogonia and primary spermatocytes throughout spermatogenesis. Moreover, adherens and hemidesmosomes junctions were observed during spermatogenesis. The above findings collectively suggest that the intensity and localization of TJ's and gap junctions vary according to the spermatogenetic stages that might be protected the developing germ cells from own immune response.