Novel Multi-isotope Tracer Approach To Test ZnO Nanoparticle and Soluble Zn Bioavailability in Joint Soil Exposures
Novel Multi-isotope Tracer Approach To Test ZnO Nanoparticle and Soluble Zn Bioavailability in Joint Soil Exposures
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DOI：
10.1021/acs.est.7b02944
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发表时间：
2017-11-07
影响因子：
11.4
通讯作者：
Rehkamper, Mark
中科院分区：
文献类型：
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作者：
Laycock, Adam;Romero-Freire, Ana;Rehkamper, Mark
在这里，我们使用两个稳定的富集态同位素，锌-68(En)和锌-(En)(~gt；99%)，制备了(氧化锌)-锌-68纳米颗粒(NPs)和可溶(氯化锌)-锌-。在鲁法2.2标准试验土壤中分别加入(氧化锌)-锌-68纳米颗粒和可溶性(氯化锌)-锌-各5 mg kg(-1)，外加天然同位素组成的可溶性氯化锌0-95 mg kg(-1)。在0、1、3、6和12个月的土壤培养后，引入蚯蚓(Eisenia Andrei)进行72h的暴露。用多收集器电感耦合等离子体质谱对土壤、孔隙水和蚯蚓组织进行分析，可以同时测量诊断的(68)锌/锌-66、锌-/锌-66和锌-68/锌-的比值，由此可以定量地确定锌的三种不同的同位素形式。在不同的总剂量浓度下，赤子爱胜蚓能够调节锌体浓度。蚯蚓对标记锌的积累与土壤中标记锌占总锌的比例成正比，且随着土壤培养时间的延长和土壤pH的降低，标记锌的积累量增加。蚯蚓(1.09+/-0.04)、土壤(1.09+/-0.02)和孔隙水(1.08+/-0.02)的锌-68(EN)/锌-(EN)比值表明，环境中锌的分布和吸收没有区别，很可能是由于氧化锌纳米颗粒的快速溶解。
Here we use two enriched stable isotopes, Zn-68(en) and Zn-64(en) (>99%), to prepare (ZnO)-Zn-68 nanoparticles (NPs) and soluble (ZnCl2)-Zn-64. The standard LUFA 2.2 test soil was dosed with (ZnO)-Zn-68 NPs and soluble (ZnCl2)-Zn-64 to 5 mg kg(-1) each, plus between 0 and 95 mg kg(-1) of soluble ZnCl2 with a natural isotope composition. After 0, 1, 3, 6, and 12 months of soil incubation, earthworms (Eisenia andrei) were introduced for 72 h exposures. Analyses of soils, pore waters, and earthworm tissues using multiple collector inductively coupled plasma mass spectrometry allowed the simultaneous measurement of the diagnostic (68)zn/Zn-66, Zn-64/Zn-66, and Zn-68/Zn-64 ratios, from which the three different isotopic forms of Zn were quantified. Eisenia andrei was able to regulate Zn body concentrations with no difference observed between the different total dosing concentrations. The accumulation of labeled Zn by the earthworms showed a direct relationship with the proportion of labeled to total Zn in the pore water, which increased with longer soil incubation times and decreasing soil pH. The Zn-68(en)/Zn-64(en) ratios determined for earthworms (1.09 +/- 0.04), soils (1.09 +/- 0.02), and pore waters (1.08 +/- 0.02) indicate indistinguishable environmental distribution and uptake of the Zn forms, most likely due to rapid dissolution of the ZnO NPs.