The creation of an infrarenal aneurysm within the native abdominal aorta of swine
The creation of an infrarenal aneurysm within the native abdominal aorta of swine
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DOI：
10.1016/j.surg.2007.04.014
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发表时间：
2007-08-01
期刊：
影响因子：
3.8
通讯作者：
Faries, Peter. L.
中科院分区：
文献类型：
--
作者：
Hynecek, Robert L.;DeRubertis, Brian G.;Faries, Peter. L.
背景。原生腹主动脉瘤（AAA）的模型已在。啮齿类动物使用弹性蛋白酶和氯化钙灌注。然而，这些模型不允许对血管内装置进行评估。本研究描述了利用机械和酶技术在猪体内产生天然AAA的方法。手术暴露的10头公约克郡猪（25-35公斤）的腹主动脉扩张，然后用50毫升的弹性蛋白酶（30单位）和胶原酶（8000单位）溶液灌注20分钟。在1、3、6周采用连续磁共振成像（AMI）评估术后主动脉直径。分别于24、48、1、2、6周实施安乐死，进行组织学观察。MRI显示主动脉平均直径增加73.3%±30.2%(33.3 ~ 116.7%)，术后逐渐增大。部分内皮细胞丢失、壁中性粒细胞浸润和弹性蛋白破坏明显（1、3和7天）。平滑肌细胞磨损发生在内膜内（7天）。3-6周出现胶原沉积、有限的SMC再生和腔内再内皮化。弹性蛋白损伤持续存在。动脉瘤形成后，主动脉直径逐渐增大是令人愉快的，需要进一步的评估来更完整地表征弹性酶诱导的猪主动脉瘤变性的性质和程度。
Background. Models of native abdominal aortic aneurysms (AAA) have been created in. rodents using elastase and calcium chloride perfusion. These models, however, do not permit the evaluation of endovascular devices. This study describes the use of mechanical and enzymatic techniques to create native AAA in swine.Methods. Surgically exposed abdominal aortas of ten male Yorkshire swine (25-35 kg) were dilated, then perfused for 20 min with a. 50-mL solution of elastase (30 units) and collagenase (8000 units). Serial magnetic resonance imaging(AMI) at 1, 3, and 6 wk was used to evaluate postoperative aortic diameter. Animals were euthanized at 24 h, 48 h and 1, 2, and 6 wk for histological evaluation.Results. MRI demonstrated an increase in mean aortic diameter by 73.3% +/- 30.2% (33.3-116.7%), which gradually increased postoperatively. Partial endothelial loss, mural neutrophil infiltrate, and elastin disruption were evident (1, 3, and 7 days). Smooth muscle cell attrition occurred within the inner tunica media (7 days). Collagen deposition, limited SMC repopulation and luminal reendothelialization appeared at 3-6 wk. Elastin injury persisted.Conclusions. The creation of an infrarenal aneurysm is possible within the native aorta of swine After aneurysm creation, progressive increase in aortic diameter was delectable Further evaluation will be necessary to more completely characterize the nature and extent of elastase-induced porcine aortic aneurysmal degeneration.