Effects of streptozotocin-induced diabetes on bladder and erectile (dys)function in the same rat in vivo
Effects of streptozotocin-induced diabetes on bladder and erectile (dys)function in the same rat in vivo
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DOI：
10.1111/j.1464-410x.2006.06058.x
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发表时间：
2006-05-01
影响因子：
4.5
通讯作者：
Melman, Arnold
中科院分区：
文献类型：
--
作者：
Christ, George J.;Hsieh, Yi;Melman, Arnold
目的建立评价糖尿病对同一只大鼠膀胱和勃起功能影响的方法、可行性和实用性，因为超过一半的糖尿病患者存在膀胱功能障碍，一半的糖尿病男性存在勃起功能障碍，但并发疾病的严重程度尚未得到严格评估。材料和方法总共有16只F-344大鼠由链脲佐菌素（STZ）诱导糖尿病，并将其分为胰岛素治疗组（5例）和未治疗组（11例），并与年龄匹配的对照组（10例）进行比较，所有这些都是平行评估的。所有STZ大鼠糖尿病8-11周。对所有大鼠进行膀胱测压研究，对一部分大鼠进行海绵体测压研究。在下列膀胱测压变量中存在胰岛素可逆性增加：膀胱重量、膀胱容量、排尿体积、残余体积、排尿压力和自发活动（P < 0.05，总体而言，单向方差分析，ANOVA）。海绵体测量显示，在所有水平的电流刺激下，海绵体神经刺激的海绵体内压（ICP）反应均出现糖尿病相关的胰岛素可逆性下降（P < 0.05，所有单因素方差分析）。绘制勃起能力（即ICP）对膀胱容量的下降程度和增加膀胱capacity. CONCLUSIONSSThese研究扩展以前的工作表明，糖尿病相关的膀胱和勃起功能障碍的程度可以在同一大鼠之间没有相关性。因此，这些发现强调了评估糖尿病对下尿路多器官系统影响的重要性。使用该模型系统的未来研究应能更好地了解这些常见糖尿病并发症的发生、发展、进展和并发症。
OBJECTIVETo establish the methods, feasibility and utility of evaluating the impact of diabetes on bladder and erectile function in the same rat, as more than half of diabetic patients have bladder dysfunction, and half of diabetic men have erectile dysfunction, but the severity of coincident disease has not been rigorously assessed.MATERIALS AND METHODSIn all, 16 F-344 rats had diabetes induced by streptozotocin (STZ), and were divided into insulin-treated (five) and untreated (11), and compared with age-matched controls (10), all assessed in parallel. All STZ rats were diabetic for 8-11 weeks. Cystometric studies were conducted on all rats, with cavernosometric studies conducted on a subset of rats.RESULTSThere were insulin-reversible increases in the following cystometric variables; bladder weight, bladder capacity, micturition volume, residual volume, micturition pressure and spontaneous activity (P < 0.05, in all, one-way analysis of variance, ANOVA). Cavernosometry showed a diabetes-related, insulin-reversible decline in the cavernosal nerve-stimulated intracavernosal pressure (ICP) response at all levels of current stimulation (P < 0.05, in all one-way ANOVA). Plotting erectile capacity (i.e. ICP) against bladder capacity showed no correlation between the extent of the decline in erectile capacity and the magnitude of the increase in bladder capacity.CONCLUSIONSThese studies extend previous work to indicate that the extent of diabetes-related bladder and erectile dysfunction can vary in the same rat. As such, these findings highlight the importance of evaluating the impact of diabetes on multiple organ systems in the lower urinary tract. Future studies using this model system should lead to a better understanding of the initiation, development, progression and coincidence of these common diabetic complications.