Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.
Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.
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缓冲梯度凝胶和 35S 标记有助于快速确定 DNA 序列。
DOI：
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发表时间：
1983
影响因子：
11.1
通讯作者：
G. Hong
中科院分区：
文献类型：
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作者：
M. Biggin;T. J. Gibson;G. Hong
描述了两种用于增加可从聚丙烯酰胺凝胶上读取的DNA序列数据长度的方法。我们开发了一种快速浇注缓冲浓度梯度凝胶的方法，通过改变放射自显像仪上的垂直条带间隔，可以从凝胶中获得更多的序列。我们还表明，在双脱氧核苷酸序列反应中使用脱氧腺苷5‘-(α-[35S]硫代)三磷酸作为标记增加了放射自显影上条带的锐度，从而提高了所达到的分辨率。
Two methods for increasing the length of DNA sequence data that can be read off a polyacrylamide gel are described. We have developed a rapid way to pour a buffer concentration gradient gel that, by altering the vertical band separation on an autoradiograph, allows more sequence to be obtained from a gel. We also show that the use of deoxyadenosine 5'-(alpha-[35S]thio)triphosphate as the label incorporated in dideoxynucleotide sequence reactions increases the sharpness of the bands on an autoradiograph and so increases the resolution achieved.