Comparison of two quantitative methods for the evaluation of neuronal number in the frontal cortex in Alzheimer disease
Comparison of two quantitative methods for the evaluation of neuronal number in the frontal cortex in Alzheimer disease
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DOI：
10.1097/00005072-199711000-00004
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发表时间：
1997-11-01
影响因子：
3.2
通讯作者：
Masliah, E
中科院分区：
文献类型：
--
作者：
Everall, IP;DeTeresa, R;Masliah, E
如何评估神经退行性疾病如阿尔茨海默病中的大量神经元损失仍在争论中。最近，体视学程序已被提出，声称提高了准确性和统计能力，但其中一些调查的结果一直存在争议。在这项研究中，我们比较和相关的细胞密度结果计算每单位体积获得的体视学技术，“选择器”，与细胞计数每单位面积获得的计算机辅助图像分析形态测量学，在相同的部分中额皮质阿尔茨海默病和对照病例。“选择器”显示神经元密度显著降低，与每单位面积大神经元计数的类似下降密切相关，如图像分析形态测定法所估计的。这些结果表明，体视学技术和图像分析形态测量学是可靠地评估神经退行性疾病中细胞群体的互补方法。
How to assess the substantial neuronal loss in a neurodegenerative disease such as Alzheimer disease is still being debated. Recently, stereological procedures have been proposed that claim improved accuracy and statistical power, but the results of some of these investigations have been controversial. In this study we compared and correlated the cell density results calculated per unit of volume obtained by a stereological technique, the ''selector,'' with the cell counts per unit area obtained by computer-aided image analysis morphometry, in the same sections of midfrontal cortex in Alzheimer disease and control cases. The ''selector'' revealed a significant decrease in neuronal density that correlated well with a similar fall in large neuronal counts per unit area, as estimated by image analysis morphometry. These results indicate that stereological techniques and image analysis morphometry are complementary methods in reliably assessing cellular populations in neurodegenerative disorders.