Regeneration of olfactory axons and synapse formation in the forebrain after bulbectomy in neonatal mice.
Regeneration of olfactory axons and synapse formation in the forebrain after bulbectomy in neonatal mice.
复制标题
新生小鼠球茎切除术后前脑嗅轴突的再生和突触的形成。
DOI：
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发表时间：
1978
影响因子：
11.1
通讯作者：
G. Graziadei
中科院分区：
文献类型：
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作者：
P. Graziadei;R. Levine;G. Graziadei
我们切除了新生小鼠的右侧嗅球，留下左侧完整的嗅球作为内部控制。在存活的第5天，我们观察到右脑半球向前移位，占据了因切除球茎而空出的区域。因此，额叶皮层离筛板很近。由于球茎消融和嗅丝分离，感觉核周发生完全逆行变性，在8天达到高峰。新的神经元在基底干细胞的神经上皮中分化，在存活30天时，成熟的感觉神经元被重建。这些新元素的轴突通过筛板到达突出的大脑半球，穿透它并直接在宿主组织中形成肾小球样结构。感觉纤维的“肾小球化”持续存在，并在60至120天内扩大。新的肾小球紧密地组织在脑组织内，皮层的大神经元被观察到在近距离。新形成肾小球的超微结构观察表明，典型的感觉轴突终末大量分支和突触，其突触后过程从周围脑组织渗透肾小球。
We removed the right olfactory bulb in neonatal mice, leaving the bulb on the left side intact as an internal control. At 5 days of survival time, we observed that the right cerebral hemisphere was displaced forward to occupy the region made vacant by removal of the bulb. The frontal cortex was, consequently, in close proximity to the lamina cribrosa. As a result of bulb ablation and severance of the fila olfactoria, the sensory perikarya underwent total retrograde degeneration, which peaked at 8 days. New neurons differentiated in the neuroepithelium from basal stem cells and, at 30 days of survival, mature sensory neurons were reconstituted. These new elements sent their axons through the lamina cribrosa to reach the protruding cerebral hemisphere, penetrating it and forming glomeruli-like structures directly in the host tissue. The "glomerulization" of the sensory fibers persisted and actually expanded between 60 and 120 days. The new glomeruli were organized intimately within the brain tissue, and large neurons of the cortex were observed to be in close proximity. Ultrastructural observations of the newly formed glomeruli demonstrated that typical sensory axon terminals profusely branched and synapsed with unidentified postsynaptic processes that penetrated the glomeruli from the surrounding cerebral tissue.