Assessment of insulin resistance in the skeletal muscle of mice using positron emission tomography/computed tomography imaging
Assessment of insulin resistance in the skeletal muscle of mice using positron emission tomography/computed tomography imaging
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使用正电子发射断层扫描/计算机断层扫描成像评估小鼠骨骼肌的胰岛素抵抗
DOI：
10.1016/j.bbrc.2020.05.165
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发表时间：
2020
影响因子：
3.1
通讯作者：
Sakaue Hiroshi
中科院分区：
文献类型：
--
作者：
Miyatake Yumiko;Mishima Yuna;Tsutsumi Rie;Otani Tamaki;Suemasa Naoya;Masumoto Saeko;Kuroda Masashi;Sakaue Hiroshi
测量体内骨骼肌的葡萄糖摄取是确定葡萄糖代谢异常的有效方法，因为骨骼肌是负责葡萄糖处理的主要组织，也是外周胰岛素抵抗的主要部位。在这项研究中，我们使用正电子发射断层扫描/计算机断层扫描（PET/CT）以无创和时间顺序的方式研究了C57BL/6J小鼠骨骼肌的病理性葡萄糖代谢动力学，这是一种利用放射性物质可视化和测量体内代谢过程的成像技术，其中[18F]-氟-2-脱氧-D-葡萄糖 （FDG）。 FDG-PET/CT 成像显示，胰岛素给药和运动负荷显着增加了 C57BL/6J 小鼠骨骼肌中 FDG 的积累。与 7 周龄 db/db 小鼠相比，表现出显着胰岛素抵抗的 14 周龄 db/db 糖尿病模型小鼠骨骼肌中 FDG 积累较低。根据对饮食诱导肥胖（DIO）小鼠体内 FDG 积累随时间变化的连续观察，17 周龄小鼠获得胰岛素抵抗后，FDG 积累显着减少。尽管在已经出现胰岛素抵抗的 20 周龄 DIO 小鼠中，骨骼肌中胰岛素诱导的葡萄糖摄取明显减弱，但运动负荷有效地增加了骨骼肌中 FDG 的摄取。因此，我们使用 PET-CT 成功证实了骨骼肌中伴随胰岛素给药和运动负荷的葡萄糖摄取增加。 FDG-PET/CT可能是一种有效的工具，可以无创地捕捉小鼠骨骼肌代谢异常的时间变化。
Measuring glucose uptake in the skeletal musclein vivois an effective method to determine glucose metabolism abnormalities as the skeletal muscle is the principal tissue responsible for glucose disposal and is a major site of peripheral insulin resistance. In this study, we investigated the pathological glucose metabolism dynamics of the skeletal muscle of C57BL/6J mice in a noninvasive and time-sequential manner using positron emission tomography/computed tomography (PET/CT), an imaging technique that uses radioactive substances to visualize and measure metabolic processes in the body, with [18F]-fluoro-2-deoxy-D-glucose (FDG). FDG-PET/CT imaging revealed that insulin administration and exercise load significantly increased FDG accumulation in the skeletal muscle of C57BL/6J mice. FDG accumulation was lower in the skeletal muscle of 14-week-olddb/dbdiabetic model mice exhibiting remarkable insulin resistance compared to that of 7-week-olddb/dbmice. Based on the continuous observation of FDG accumulation over time in diet-induced obese (DIO) mice, FDG accumulation significantly decreased in 17-week-old mice after the acquisition of insulin resistance. Although insulin-induced glucose uptake in the skeletal muscle was markedly attenuated in 20-week-old DIO mice that had already developed insulin resistance, exercise load effectively increased FDG uptake in the skeletal muscle. Thus, we successfully confirmed that glucose uptake accompanied by insulin administration and exercise load increased in the skeletal muscle using PET-CT. FDG-PET/CT might be an effective tool that could noninvasively capture the chronological changes of metabolic abnormalities in the skeletal muscle of mice.