The plasma membrane-actin linking protein, ezrin, is a glomerular epithelial cell marker in glomerulogenesis, in the adult kidney and in glomerular injury
The plasma membrane-actin linking protein, ezrin, is a glomerular epithelial cell marker in glomerulogenesis, in the adult kidney and in glomerular injury
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DOI：
10.1046/j.1523-1755.1998.00195.x
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发表时间：
1998-12-01
影响因子：
19.6
通讯作者：
Johnson, RJ
中科院分区：
文献类型：
--
作者：
Hugo, C;Nangaku, M;Johnson, RJ
背景。 Ezrin 属于质膜-细胞骨架连接肌动蛋白结合蛋白家族（Ezrin-radixin-Moesin 家族），参与信号转导、生长控制、细胞间粘附和微绒毛形成。方法。在培养的肾小球细胞、肾脏发育期间、成熟肾脏以及大鼠五种不同的实验性肾脏疾病模型中检查了埃兹蛋白的表达。结果。 Ezrin在发育中的肾小球、成熟的肾小球和培养的肾小球上皮细胞中的肾小球上皮细胞中特异性表达。与主要在肾小球内皮和系膜区域表达的其他家族成员、moesin 和 radixin 不同，ezrin 蛋白（通过免疫组织化学）在足细胞损伤和再生过程中受到特异性和排他性调节。 Ezrin 免疫组织化学能够观察细胞体衰减、假性囊肿，特别是受损足细胞的空泡形成，这一特征通常必须在超微结构水平上进行识别，并且在双核足细胞或部分或完全与底层 GEM 分离的足细胞（通常也是双核）中显着增加。浸润性巨噬细胞也表达埃兹蛋白，但由于其圆形和较高的表达水平，很容易与足细胞区分开来。结论。埃兹蛋白可能在细胞骨架组织中发挥作用，例如伴随足细胞损伤和再生的肌动蛋白丝的重新组装。由于用于识别肾小球上皮细胞的合适光学显微镜标记物很少，因此埃兹蛋白也可能是正常和受损肾小球中足细胞的有用标记物。
Background. Ezrin belongs to a family of plasma membrane-cytoskeleton linking, actin binding proteins (Ezrin-radixin-Moesin family) involved in signal transduction, growth control, cell-cell adhesion, and microvilli formation.Methods. The expression of ezrin was examined in glomerular cells in culture, during kidney development, in the mature kidney, and in five different experimental kidney disease models in the rat.Results. Ezrin was specifically expressed in glomerular epithelial cells in developing glomeruli in mature glomeruli and in glomerular epithelial cells in culture. Distinct from its other family members, moesin and radixin, which are predominantly expressed in glomerular endothelial and mesangial areas, ezrin protein (by immunohistochemistry) was specifically and exclusively modulated during podocyte injury and regeneration. Ezrin immunohistochemistry was able to visualize cell body attenuation, pseudocysts, and in particular vacuolation of injured podocytes, a feature that usually has to be identified at the ultrastructural level, and was strikingly increased in binucleated podocytes or podocytes that were partially or completely detached from the underlying GEM (frequently also binucleated). Infiltrating macrophages also express ezrin, but can easily be differentiated from podocytes by their round shape and higher level of expression.Conclusions. Ezrin likely has a role in the cytoskeletal organization, such as reassembling of actin filaments accompanying podocyte injury and regeneration. Since suitable light microscopic markers for the identification of glomerular epithelial cells are rare, ezrin may also be a useful marker for podocytes in normal and injured glomeruli.