Microarray-based identification of lectins for the purification of human urinary extracellular vesicles directly from urine samples.
Microarray-based identification of lectins for the purification of human urinary extracellular vesicles directly from urine samples.
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DOI：
10.1002/cbic.201402058
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发表时间：
2014-07-21
期刊：
影响因子：
--
通讯作者：
Reichardt, Niels-Christian
中科院分区：
文献类型：
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作者：
Echevarria, Juan;Royo, Felix;Reichardt, Niels-Christian
由于细胞衍生的囊泡在很大程度上保持了其原始组织的生物分子组成，因此几乎在所有体液中发现的外泌体作为临床疾病标记物具有巨大的潜力。开发基于外泌体的诊断测定的主要瓶颈是这些囊泡的具有挑战性的纯化;这需要耗时且基于仪器的程序。我们采用凝集素阵列，以确定潜在的凝集素作为探针的亲和力为基础的分离外泌体从尿基质。我们发现了三种凝集素，表现出特定的相互作用，囊泡和没有（或只有残留）与基质蛋白的相互作用。基于这些发现，开发了一种用于从尿液中基于凝集素分离外来体的基于珠的方法，作为基于外来体的生物标志物研究的样品制备步骤。
As cellular-derived vesicles largely maintain the biomolecule composition of their original tissue, exosomes, which are found in nearly all body fluids, have enormous potential as clinical disease markers. A major bottleneck in the development of exosome-based diagnostic assays is the challenging purification of these vesicles; this requires time-consuming and instrument-based procedures. We employed lectin arrays to identify potential lectins as probes for affinity-based isolation of exosomes from the urinary matrix. We found three lectins that showed specific interactions to vesicles and no (or only residual) interaction with matrix proteins. Based on these findings a bead-based method for lectin-based isolation of exosomes from urine was developed as a sample preparation step for exosome-based biomarker research.