Evaluation of distinct blood lymphocyte populations in human immunodeficiency virus type 1-infected subjects in the absence or presence of effective therapy
Evaluation of distinct blood lymphocyte populations in human immunodeficiency virus type 1-infected subjects in the absence or presence of effective therapy
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DOI：
10.1086/315117
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发表时间：
1999-12-01
影响因子：
6.4
通讯作者：
Bucy, RP
中科院分区：
文献类型：
--
作者：
Derdeyn, CA;Kilby, JM;Bucy, RP
尽管有效抑制血浆病毒，但病毒库仍可在人类免疫缺陷病毒 1 型 (HIV-1) 感染受试者中持续存在。为了比较在不存在和存在抗逆转录病毒治疗的情况下的病毒动力学，通过使用原位杂交检查了 19 名血浆 RNA 水平高的受试者和 18 名长期病毒抑制受试者的血液单核细胞，以检测病毒 RNA 表达 体外 T 细胞激活前后。这种方法揭示了在激活前表达 HIV-1 RNA 的循环淋巴细胞，以及在体外激活后具有可检测到的 HIV-1 RNA 转录的细胞的增加。这两种细胞群的频率与血浆病毒载量密切相关，并且一旦在治疗期间建立新的稳定状态，它们似乎就稳定了。病毒RNA阳性细胞的频率与产生感染性病毒的细胞的频率相当。因此，在HIV-1感染的受试者中，存在包含潜伏细胞和复制活性细胞的不同病毒储存库。
Virus reservoirs can persist in human immunodeficiency virus type 1 (HIV-l)-infected subjects despite effective plasma virus suppression, To compare viral dynamics in the absence and presence of antiretroviral therapy, blood mononuclear cells from 19 subjects with high plasma RNA levels and 18 subjects following prolonged virus suppression were examined, by use of in situ hybridization, to detect virus RNA expression before and after in vitro T cell activation. This approach reveals circulating lymphocytes expressing HIV-1 RNA before activation and an increase in cells with detectable HIV-1 RNA transcription after in vitro activation. The frequencies of these 2 cell populations are strongly correlated with plasma virus load and appear to be stable once a new steady state is established during therapy. The frequency of viral RNA-positive cells is equivalent to the frequency of cells that produce infectious virus. Thus, in HIV-l-infected subjects there are distinct virus reservoirs comprising both latent and replication-active cells.