A brief introduction to tiling microarrays: principles, concepts, and applications.
A brief introduction to tiling microarrays: principles, concepts, and applications.
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DOI：
10.1007/978-1-62703-607-8_1
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发表时间：
2013
影响因子：
--
通讯作者：
C. Lemetre;Zhengdong D. Zhang
中科院分区：
文献类型：
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作者：
C. Lemetre;Zhengdong D. Zhang
技术成果一直为生物医学研究的进步做出贡献。最近，创新尖端技术的开发和应用将生物学转变为数据丰富的定量科学，这一点从未如此。这场令人震惊的生物学革命主要源于二十多年前微阵列的出现。全基因组测序项目的完成和微阵列制造技术的进步促进了平铺微阵列的发展，它提供了前所未有的基因组覆盖范围。自从首次描述以来，已经出现了几种类型的平铺阵列应用，每种类型都旨在解决不同的生物学问题。尽管已经开发出许多算法来分析微阵列数据，但仍然需要开发新的方法，不仅是为了更好的性能，而且是为了整合可用的微阵列数据集，这无疑构成了有史以来最大的生物数据集之一。在本章中，我们首先介绍平铺微阵列的出现和发展背后的原理，然后通过一些例子讨论它们如何用于研究不同的生物学问题。
Technological achievements have always contributed to the advancement of biomedical research. It has never been more so than in recent times, when the development and application of innovative cutting-edge technologies have transformed biology into a data-rich quantitative science. This stunning revolution in biology primarily ensued from the emergence of microarrays over two decades ago. The completion of whole-genome sequencing projects and the advance in microarray manufacturing technologies enabled the development of tiling microarrays, which gave unprecedented genomic coverage. Since their first description, several types of application of tiling arrays have emerged, each aiming to tackle a different biological problem. Although numerous algorithms have already been developed to analyze microarray data, new method development is still needed not only for better performance but also for integration of available microarray data sets, which without doubt constitute one of the largest collections of biological data ever generated. In this chapter we first introduce the principles behind the emergence and the development of tiling microarrays, and then discuss with some examples how they are used to investigate different biological problems.