Surface Coatings Modulate the Differences in the Adhesion Forces of Eukaryotic and Prokaryotic Cells as Detected by Single Cell Force Microscopy
Surface Coatings Modulate the Differences in the Adhesion Forces of Eukaryotic and Prokaryotic Cells as Detected by Single Cell Force Microscopy
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DOI：
10.1155/2019/7024259
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发表时间：
2019-04
影响因子：
3.1
通讯作者：
Philipp Wysotzki;J. Gimsa
中科院分区：
文献类型：
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作者：
Philipp Wysotzki;J. Gimsa
单细胞力显微镜用于研究原代神经元小鼠细胞（PNC）、成骨细胞（MC 3 T3）和原核细胞（头葡萄球菌亚种）的最大脱离力（MST）。capitis）从不同的表面分离。用带正电荷的聚乙烯亚胺（PEI）或聚-D-赖氨酸涂覆带正电荷的氮化硅表面。层粘连蛋白用作第二涂层。PEI诱导的MDF的数量级为5至20 nN，略高于氮化硅。在PEI/层粘连蛋白上检测到较低的MDF（1至5 nN），在PDL/层粘连蛋白上检测到最低。为了从单个电池特性（例如尺寸）中提取并获得电池类型特异性的MDF，将不同涂层上的每个电池的MDF归一化为氮化硅参比物的最长接触时间。原核细胞和真核细胞之间的差异通常具有相似的尺寸，除了PDL/层粘连蛋白，其区别于原核细胞。我们解释了较低的MDF层粘连蛋白的静电细胞粘附到底层聚合物的空间预防。然而，PEI可以形成从表面结合层突出的长的柔性环，其可以跨越层粘连蛋白层并且容易地结合到细胞表面和小的原核细胞。这反映在氮化硅上两秒接触时间后MDF增加，而在PEI或PEI/层粘连蛋白上一秒后已经观察到两秒值。我们假设，与PEI环的静电荷相互作用是更重要的较小的原核细胞比真核细胞的初始粘附。
Single cell force microscopy was used to investigate the maximum detachment force (MDF) of primary neuronal mouse cells (PNCs), osteoblastic cells (MC3T3), and prokaryotic cells (Staphylococcus capitis subsp. capitis) from different surfaces after contact times of 1 to 5 seconds. Positively charged silicon nitride surfaces were coated with positively charged polyethyleneimine (PEI) or poly-D-lysine. Laminin was used as the second coating. PEI induced MDFs of the order of 5 to 20 nN, slightly higher than silicon nitride did. Lower MDFs (1 to 5 nN) were detected on PEI/laminin with the lowest on PDL/laminin. To abstract from the individual cell properties, such as size, and to obtain cell type-specific MDFs, the MDFs of each cell on the different coatings were normalized to the silicon nitride reference for the longest contact time. The differences in MDF between prokaryotic and eukaryotic cells were generally of similar dimensions, except on PDL/laminin, which discriminated against the prokaryotic cells. We explain the lower MDFs on laminin by the spatial prevention of the electrostatic cell adhesion to the underlying polymers. However, PEI can form long flexible loops protruding from the surface-bound layer that may span the laminin layer and easily bind to cellular surfaces and the small prokaryotic cells. This was reflected in increased MDFs after two-second contact times on silicon nitride, whereas the two-second values were already observed after one second on PEI or PEI/laminin. We assume that the electrostatic charge interaction with the PEI loops is more important for the initial adhesion of the smaller prokaryotic cells than for eukaryotic cells.