Platycodin D2 is a potential less hemolytic saponin adjuvant eliciting Th1 and Th2 immune responses
Platycodin D2 is a potential less hemolytic saponin adjuvant eliciting Th1 and Th2 immune responses
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Platycodin D2 是一种潜在的溶血性较低的皂苷佐剂，可引发 Th1 和 Th2 免疫反应
DOI：
10.1016/j.intimp.2008.04.006
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发表时间：
2008-08-01
影响因子：
5.6
通讯作者：
Li, Duo
中科院分区：
文献类型：
--
作者：
Xie, Yong;Deng, Wen;Li, Duo
桔梗皂苷D2是从桔梗根部分离得到的一种皂苷，对其溶血活性和对卵清蛋白(OVA)诱导的小鼠细胞和体液免疫应答的佐剂作用进行了研究。PD2和QUIT A对0.5%兔红细胞的HD(50)值分别为18.57+/-1.37和5.76+/-0.23 mU/ml，提示PD2的溶血活性低于QUT A。ICR小鼠分别皮下注射OVA 100 mU g、OVA 100 mG溶于含明矾的生理盐水200 mU g或PD2(25、50、75或100 mU g)。检测内毒素(LPS)和卵清蛋白(OVA)刺激的脾细胞增殖反应及血清中的OVA特异性抗体。PD2能显著增强ConA、LPS和OVA诱导的OVA免疫小鼠脾细胞增殖反应(P
Platycodin D2 (PD2), a saponin isolated from the root of Platycodon grandiflorum, was evaluated for its haemolytic activity and adjuvant potential on the cellular and humoral immune responses to ovalbumin (OVA) in ICR mice. The HD(50) values of PD2 and Quit A were 18.57 +/- 1.37 and 5.76 +/- 0.23 mu g/ml against 0.5% rabbit red blood cell, respectively, implicating the less haemolytic activity for PD2 than Quit A. ICR mice were immunized subcutaneously with OVA 100 mu g alone or with OVA 100 mu g dissolved in saline containing Alum (200 mu g), or PD2 (25, 50, 75 or 100 mu g) on Day 1 and 15. Two weeks later (Day 28), concanavalin A (Con A)-, lipopolysaccharide (LPS-) and OVA-stimulated splenocyte proliferation and OVA-specific antibodies in serum were measured. PD2 significantly enhanced the Con A-, LPS-, and OVA-induced splenocyte proliferation in the OVA-immunized mice (P