BAFILOMYCINS - A CLASS OF INHIBITORS OF MEMBRANE ATPASES FROM MICROORGANISMS, ANIMAL-CELLS, AND PLANT-CELLS
BAFILOMYCINS - A CLASS OF INHIBITORS OF MEMBRANE ATPASES FROM MICROORGANISMS, ANIMAL-CELLS, AND PLANT-CELLS
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DOI：
10.1073/pnas.85.21.7972
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发表时间：
1988-11-01
影响因子：
11.1
通讯作者：
ALTENDORF, K
中科院分区：
文献类型：
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作者：
BOWMAN, EJ;SIEBERS, A;ALTENDORF, K
已经测试了各种膜ATP酶对大环内酯类抗生素巴弗洛霉素A1的敏感性。来自细菌和线粒体的F1 F0 ATP酶不受这种抗生素的影响。相反，E1 E2 ATP酶，例如，来自大肠杆菌的K+依赖性（Kdp）ATP酶、来自牛脑的Na+，K+-ATP酶和来自肌质网的Ca 2 +-ATP酶对该抑制剂中等敏感。最后，来自脉孢菌液泡、嗜铬颗粒和植物液泡的膜ATP酶非常敏感。从这一点，我们得出结论，巴弗洛霉素A1是一个有价值的工具，区分三种不同类型的ATP酶，并代表了第一个相对具体的有效抑制剂液泡ATP酶。
Various membrane ATPases have been tested for their sensitivity to bafilomycin A1, a macrolide antibiotic. F1F0 ATPases from bacteria and mitochondria are not affected by this antibiotic. In contrast, E1E2 ATPases.sbd.e.g., the K+-dependent (Kdp) ATPase from Escherichia coli, the Na+, K+-ATPase from ox brain, and the Ca2+-ATPase from sarcoplasmic reticulum.sbd.are moderately sensitive to this inhibitor. Finally, membrane ATPases from Neurospora vacuoles, chromaffin granules, and plant vacuoles are extremely sensitive. From this we conclude that bafilomycin A1 is a valuable tool for distinguishing among the three different types of ATPases and represents the first relatively specific potent inhibitor of vacuolar ATPases.