Evaluation of the COBAS TaqMan 48 real-time PCR system for quantitation of hepatitis B virus DNA
Evaluation of the COBAS TaqMan 48 real-time PCR system for quantitation of hepatitis B virus DNA
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DOI：
10.1128/jcm.43.7.3504-3507.2005
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发表时间：
2005-07-01
影响因子：
9.4
通讯作者：
Casal, M
中科院分区：
文献类型：
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作者：
Gordillo, RM;Gutiérrez, J;Casal, M
本研究的目的是评价新型实时PCR COBAS TaqMan 48分析仪的有效性，并将其与现有的基于传统PCR技术的COBAS AMPLYCOR HBV MONITOR进行比较。该研究使用了来自不同患者的104个样本。在测试的灵敏度方面没有发现差异。在两次检测的动态范围内，病毒载量样本之间存在良好的相关性（r = 0.938）。COBAS TaqMan测试具有更宽的线性范围，这一事实使得无需稀释样品即可定量病毒载量。
The purpose of this study is to evaluate the usefulness of the new real-time PCR COBAS TaqMan 48 analyzer, comparing it to the existing COBAS AMPLICOR HBV MONITOR based on conventional PCR technology. The study used 104 samples from different patients. No differences were found in the sensitivity of the tests. There was an excellent correlation between the sample with a viral load within the dynamic range of the two tests (r = 0.938). The COBAS TaqMan test has a wider linear range, and this fact enables quantifying of the viral load without diluting the sample.