Analysis of Physcomitrella Phytochrome Mutants via Phototropism and Polarotropism.

Analysis of Physcomitrella Phytochrome Mutants via Phototropism and Polarotropism.
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通过向光性和极向性分析立碗藻光敏色素突变体

DOI:
10.1007/978-1-4939-9612-4_19
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发表时间:
2019
影响因子:
--
通讯作者:
Hughes J
Hughes J
中科院分区:
--
文献类型:
--
作者:
Ermert AL;Stahl F;Gans T;Hughes J

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在苔藓中,如立碗藓(Physcomitrella patensphytochrome photoreceptors),光感受器对单侧/偏振光进行方向性/矢量性反应。在这一章中,我们描述了定量测定野生型和突变株系向光性和向极性的方法。在黑暗中将原丝体置于方形培养皿中的琼脂基培养基上1周,使茎丝体发育并负向重力生长。对于向光性,将培养皿垂直放置在黑盒子中,并用连续红光单侧照射。对于向极性,将培养皿水平放置并从上方用线性偏振红光照射。照射后,使用具有CCD相机的宏观镜对长丝拍照,并使用图像处理软件测量弯曲角度。将数据传输到电子表格程序中,放入10°弯曲角度类中,并使用圆形直方图进行说明。
In mosses such asPhyscomitrella patensphytochrome photoreceptors steer directional/vectorial responses to unilateral/polarized light. In this chapter, we describe procedures to assay phototropism and polarotropism quantitatively in wild type and mutant lines. Protonemata are placed on agar-based medium in square Petri dishes in darkness for 1 week, allowing caulonemata to develop and grow negatively gravitropically. For phototropism, the dishes are placed vertically in black boxes and unilaterally irradiated with continuous red light. For polarotropism, Petri dishes are placed horizontally and irradiated with linearly polarized red light from above. After irradiation, the filaments are photographed using a macroscope with CCD camera and the bending angles measured using image processing software. The data are transfered to a spreadsheet program, placed into 10° bending angle classes and illustrated using a circular histogram.
DOI: --
发表时间: 1935
期刊:
影响因子: --
作者:
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