Analysis of Physcomitrella Phytochrome Mutants via Phototropism and Polarotropism.
Analysis of Physcomitrella Phytochrome Mutants via Phototropism and Polarotropism.
复制标题
通过向光性和极向性分析立碗藻光敏色素突变体
DOI:
10.1007/978-1-4939-9612-4_19
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发表时间:
2019
影响因子:
--
通讯作者:
Hughes J
中科院分区:
文献类型:
--
作者:
Ermert AL;Stahl F;Gans T;Hughes J
In mosses such asPhyscomitrella patensphytochrome photoreceptors steer directional/vectorial responses to unilateral/polarized light. In this chapter, we describe procedures to assay phototropism and polarotropism quantitatively in wild type and mutant lines. Protonemata are placed on agar-based medium in square Petri dishes in darkness for 1 week, allowing caulonemata to develop and grow negatively gravitropically. For phototropism, the dishes are placed vertically in black boxes and unilaterally irradiated with continuous red light. For polarotropism, Petri dishes are placed horizontally and irradiated with linearly polarized red light from above. After irradiation, the filaments are photographed using a macroscope with CCD camera and the bending angles measured using image processing software. The data are transfered to a spreadsheet program, placed into 10° bending angle classes and illustrated using a circular histogram.
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DOI:
--
发表时间:
1935
期刊:
影响因子:
--
作者:
L. H. Flint;E. D. Mcalister
通讯作者:
E. D. Mcalister
DOI:
--
发表时间:
2004
影响因子:
11.1
作者:
Emmanuelle Monte
通讯作者:
Emmanuelle Monte
DOI:
--
发表时间:
1991
影响因子:
11.1
作者:
A. Nagatani;S. Kay;M. Deák;N. Chua;M. Furuya
通讯作者:
M. Furuya
影响因子:
2.9
作者:
MESKE, V;HARTMANN, E
通讯作者:
HARTMANN, E
影响因子:
7.2
作者:
Tepperman, James M.;Hwang, Yong-Sic;Quail, Peter H.
通讯作者:
Quail, Peter H.