The immunological footprint of CMV in HIV-1 patients stable on long-term ART.
The immunological footprint of CMV in HIV-1 patients stable on long-term ART.
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DOI：
10.1186/s12979-015-0041-0
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发表时间：
2015
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影响因子：
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通讯作者：
Price P
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作者：
Affandi JS;Montgomery J;Brunt SJ;Nolan D;Price P
大多数艾滋病毒感染者巨细胞病毒（CMV）血清呈阳性，并保留潜伏病毒，可通过免疫激活重新激活。它们的 T 细胞群表达反映分化后期的标记，但 HIV 和 CMV 对这一特征的贡献尚不清楚。我们调查了 HIV 患者中 CMV 的免疫“足迹”，这些患者有严重免疫缺陷病史，但目前通过抗逆转录病毒治疗 (ART) 病情稳定。在接受 ART 治疗超过 12 年后，对 20 名年龄 >50 岁且最低 CD4 T 细胞计数 <200 个细胞/μl 的 CMV 血清阳性 HIV 患者进行了研究。包括 16 名 CMV 血清阳性和 9 名 CMV 血清阴性健康对照。 HIV患者的CMV抗体滴度高于对照组（P<0.001-0.003）。患者中可溶性 B 细胞激活因子 (sBAFF) 水平升高 (P = 0.002)，并与 CMV 抗体水平相关 (P = 0.03-0.002)，而对照组则没有明确的关系。 HIV 患者中 CD8 T 细胞 IFNγ 对 IE1 肽 (VLE) 的反应仍然升高 (P = 0.005)。 CD8 T 细胞的 CD57+CD45RA+CD27− 表型与年龄相关 (r = 0.60, P = 0.006)、抗 CMV IE1 蛋白抗体 (r = 0.44, P = 0.06) 和 CD4 T 细胞 IFNγ 对 CMV 裂解物的反应（r = 0.45，P = 0.05）。在接受 ART 超过 12 年之后，HIV 患者的体液和 T 细胞对 CMV 的反应仍然较高。年龄和 CMV 疾病的存在影响 CD8 T 细胞表型。 sBAFF 水平升高可能是 HIV 疾病的结果，并导致 CMV 抗体滴度升高。本文的在线版本 (doi:10.1186/s12979-015-0041-0) 包含补充材料，可供授权用户使用。
Most HIV-infected persons are cytomegalovirus (CMV) seropositive and retain latent virus that can be reactivated by immune activation. Their T cell populations express markers reflecting a late stage of differentiation, but the contributions of HIV and CMV to this profile are unclear. We investigated the immunological “footprint” of CMV in HIV patients who had a history of extreme immunodeficiency but were now stable on antiretroviral therapy (ART). Twenty CMV seropositive HIV patients >50 years old with nadir CD4 T-cell counts <200 cells/μl were studied after >12 years on ART. 16 CMV seropositive and 9 CMV seronegative healthy controls were included. CMV antibody titres were higher in HIV patients than controls (P < 0.001-0.003). Levels of soluble B-cell activating factor (sBAFF) were elevated in patients (P = 0.002) and correlated with levels of CMV antibodies (P = 0.03-0.002), with no clear relationship in controls. CD8 T-cell IFNγ responses to the IE1 peptide (VLE) remained elevated in HIV patients (P = 0.005). The CD57+CD45RA+CD27− phenotype of CD8 T-cells correlated with age (r = 0.60, P = 0.006), antibodies against CMV IE1 protein (r = 0.44, P = 0.06) and CD4 T-cell IFNγ response to CMV lysate (r = 0.45, P = 0.05). Humoral and T-cell responses to CMV remained elevated in HIV patients after >12 years on ART. Age and presence of CMV disease influenced CD8 T-cell phenotypes. Elevated levels of sBAFF may be a consequence of HIV disease and contribute to high titres of CMV antibody. The online version of this article (doi:10.1186/s12979-015-0041-0) contains supplementary material, which is available to authorized users.