Fatty acid composition of lipids which copurify with band 3.
Fatty acid composition of lipids which copurify with band 3.
复制标题
与带 3 共纯化的脂质的脂肪酸组成。
DOI：
10.1016/0006-291x(89)92209-2
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发表时间：
1989
影响因子：
3.1
通讯作者：
Low,PS
中科院分区：
文献类型：
--
作者：
Maneri,LR;Low,PS
在先前的研究中（LR Maneri和PS Low（1988）J.Biol.Chem.263，16170-16178），我们确定阴离子转运蛋白带3被含有饱和和/或长链脂肪酸的脂质显著稳定。为了确定这种热力学偏好是否反映在与体内阴离子转运蛋白紧密相关的脂质组合物中，我们分析了与带3的纯化的积分域共分离的磷脂的脂肪酸含量。我们的数据表明，尽管硬脂酸仅占红细胞膜的主体脂质脂肪酸的14%，但其占与条带3共分离的脂质的脂肪酸的约68%。某些其他长链脂肪酸也富集在粘附脂质中。这些结果表明，最有效地稳定条带3的脂肪酸也对转运蛋白具有最高的亲和力。
In a previous study (LR Maneri and PS Low (1988) J. Biol. Chem. 263, 16170–16178) we determined that the anion transport protein, band 3, was significantly stabilized by lipids containing saturated and/or long chain fatty acids. To determine whether this thermodynamic preference is reflected in the composition of lipids tightly associating with the anion transporter in vivo, we have analyzed the fatty acid content of phospholipids co-isolating with the purified integral domain of band 3. Our data demonstrate that although stearic acid comprises only 14% of the bulk lipid fatty acids of the red cell membrane, it constitutes∼ 68% of the fatty acids of lipids co-isolating with band 3. Certain other long chain fatty acids were also enriched in the adherent lipids. These results suggest that the fatty acids which most effectively stabilize band 3 also have the highest affinity for the transport protein.