Mammalian type I interferon receptors consists of two subunits: IFNaR1 and IFNaR2
Mammalian type I interferon receptors consists of two subunits: IFNaR1 and IFNaR2
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DOI：
10.1016/s0378-1119(97)00240-0
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发表时间：
1997-09-01
期刊：
影响因子：
3.5
通讯作者：
Rubinstein, M
中科院分区：
文献类型：
--
作者：
Kim, SH;Cohen, B;Rubinstein, M
人I型干扰素（IFN）受体由两个基本亚基huIFNaR 1和huIFNaR 2组成;然而，到目前为止，在其他物种中仅鉴定出IFNaR 1。此外，已经表明，在某些物种中，I型IFN受体可能由单个亚基组成，因为鼠IFNaR 1在人细胞中的表达使它们对几种I型鼠IFN产生应答。为了解决这个问题，我们用来自huIFNaR 2 cDNA的探针筛选小鼠cDNA文库。分离了编码与huIFNaR 2具有49%同一性的跨膜蛋白的cDNA克隆。这种同一性水平表明该cDNA编码muIFNaR 2。此外，几个cDNA克隆，编码muIFNaR 2的两个不同的可溶性变体，进行了鉴定。为了测试muIFNaR 2是否是受体的功能组分，我们将其与muIFNaR 1在人细胞中共表达，并与IFN响应性荧光素酶报告载体共表达。用muIFN-β处理这些细胞诱导高水平的荧光素酶，而在仅表达两种亚基之一的细胞中没有获得诱导。因此，我们得出结论，小鼠I型IFN受体由两个不同的亚基-一个配置共享的人类，可能所有其他哺乳动物。(C)1997年Elsevier Science B.V.
The human type I interferon (IFN) receptor consists of two essential subunits, huIFNaR1 and huIFNaR2; however, so far only IFNaR1 has been identified in other species. Furthermore, it has been suggested that in some species the type I IFN receptor may consist of a single subunit, since expression of murine IFNaR1 in human cells rendered them responsive to several type I murine IFNs. To resolve this issue, we screened a mouse cDNA library with a probe derived from huIFNaR2 cDNA. A cDNA clone, coding for a transmembrane protein which has 49% identity with huIFNaR2 was isolated. This level of identity suggests that this cDNA codes for a muIFNaR2. In addition, several cDNA clones, coding for two distinct soluble variants of muIFNaR2, were identified. To test whether muIFNaR2 is a functional component of the receptor, we co-expressed it with muIFNaR1 in human cells and with an IFN-responsive luciferase reporter vector. Treatment of these cells with muIFN-beta induced high levels of luciferase, whereas no induction was obtained in cells expressing only one of the two subunits. We therefore conclude that the murine type I IFN receptor consists of two different subunits - a configuration shared by humans, and probably all other mammals. (C) 1997 Elsevier Science B.V.