Efficient Incorporation of Free Oxygen into Volicitin in Spodoptera litura Common Cutworm Larvae

Efficient Incorporation of Free Oxygen into Volicitin in Spodoptera litura Common Cutworm Larvae
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斜纹夜蛾幼虫中自由氧与 Volicitin 的有效结合

DOI:
10.1271/bbb.90207
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发表时间:
2009
期刊:
Bioscience, Biotechnology, and Biochemistry
影响因子:
--
通讯作者:
N. Mori
N. Mori
中科院分区:
--
文献类型:
--
作者:
Chihiro Ishikawa;N. Yoshinaga;Takako Aboshi;R. Nishida;N. Mori

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Volicitin [N-(17-羟基亚麻烯酰)- l -谷氨酰胺]先前已从鳞翅目幼虫反刍物中鉴定出作为植物挥发性排放物的激发物。斜纹夜蛾幼虫在18O2下饲养3天,证明了游离氧有效地结合到volicitin中。利用液相色谱/质谱-离子捕获飞行时间(LC/MS-IT-TOF)技术证实了莲子素羟基的18o标记,并提示了莲子素生物合成过程中单加氧酶的活性。
Volicitin [N-(17-hydroxylinolenoyl)-L-glutamine] has previously been identified from the lepidopteran larval regurgitant as an elicitor of plant volatile emission. The efficient incorporation of free oxygen into volicitin by Spodoptera litura larvae is demonstrated here by rearing them under 18O2 for three days. 18O-labeling of the hydroxyl group of volicitin was confirmed by liquid chromatography/mass spectrometry-ion trap-time-of-flight (LC/MS-IT-TOF) and suggests the activity of a monooxygenase in volicitin biosynthesis.
DOI: 10.1271/bbb.60133
发表时间: 2006-09-01
影响因子: 1.6
作者:
Sawada, Yoshitsugu;Yoshinaga, Naoko;Mori, Naoki
通讯作者: Mori, Naoki