Nervous system targets of RNA editing identified by comparative genomics
Nervous system targets of RNA editing identified by comparative genomics
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DOI：
10.1126/science.1086763
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发表时间：
2003-08-08
期刊：
影响因子：
56.9
通讯作者：
Reenan, R
中科院分区：
文献类型：
--
作者：
Hoopengardner, B;Bhalla, T;Reenan, R
莫迪对数量不详的前体信使RNA进行了基因重新编码。腺苷到肌苷的阳离子，由作用于RNA的腺苷脱氨酶(ADARs)催化的反应。这些经过编辑的文字记录的发现一直是偶然的。利用比较基因组学，我们确定了RNA编辑的系统发育特征。我们报告了鉴定和实验验证。果蝇中16个以前未知的ADAR靶基因中的阳离子，以及人类中的1个--超过了之前报道的总和。所有这些基因都参与了快速的电子和化学神经传递，许多编辑过的位点重新编码了保守的、具有重要功能的氨基酸。这些结果表明了RNA编辑在神经系统功能中的关键作用。
An unknown number of precursor messenger RNAs undergo genetic recoding by modi. cation of adenosine to inosine, a reaction catalyzed by the adenosine deaminases acting on RNA(ADARs). Discovery of these edited transcripts has always been serendipitous. Using comparative genomics, we identified a phylogenetic signature of RNA editing. We report the identification and experimental veri. cation of 16 previously unknown ADAR target genes in the fruit fly Drosophila and one in humans - more than the sum total previously reported. All of these genes are involved in rapid electrical and chemical neurotransmission, and many of the edited sites recode conserved and functionally important amino acids. These results point to a pivotal role for RNA editing in nervous system function.