Thin Frozen Film Method for Visualization of Storage Proteins in Mature Rice Grains
Thin Frozen Film Method for Visualization of Storage Proteins in Mature Rice Grains
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DOI：
10.1271/bbb.80337
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发表时间：
2008-10-01
影响因子：
1.6
通讯作者：
Masumura, Takehiro
中科院分区：
文献类型：
--
作者：
Saito, Yuhi;Nakatsuka, Nobuaki;Masumura, Takehiro
要获得保存完好的成熟细胞及其亚细胞结构的完整谷物切片，存在技术上的困难。在这里，我们描述了一种简单的切开硬质成熟稻谷的方法。这使得在水稻胚乳的高质量组织切片中准确定位贮藏蛋白成为可能。
There are technical difficulties in obtaining intact sections of cereal grains in which mature cells and their subcellular structures are well preserved. Here we describe a simple method for sectioning hard mature rice grains. It makes possible accurate localization of storage proteins in high-quality histological sections of rice endosperm.