Salicylic acid and broad spectrum of NBS-LRR family genes are involved in SMV-soybean interactions.
Salicylic acid and broad spectrum of NBS-LRR family genes are involved in SMV-soybean interactions.
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DOI：
10.1016/j.plaphy.2017.12.011
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发表时间：
2018-02
期刊：
影响因子：
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通讯作者：
Qiqi Zhao;Haina Li;Hongyu Sun;Aoga Li;Shuxin Liu;Ruonan Yu;X. Cui;Dejian Zhang;H. Wuriyanghan
中科院分区：
文献类型：
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作者：
Qiqi Zhao;Haina Li;Hongyu Sun;Aoga Li;Shuxin Liu;Ruonan Yu;X. Cui;Dejian Zhang;H. Wuriyanghan
大豆花叶病毒（SMV）是一种严重影响大豆产量和种子品质的病原菌。虽然在一些大豆品种中发现了grsv1、Rsv3和rsv4等抗性基因位点，但大多数与SMV感染相关的大豆基因仍未被鉴定。为了揭示SMV感染反应的全基因组基因表达谱，我们利用转录组分析确定了敏感品种和丰25的SMV应答基因。时间过程RNA-seq分析在1,5和10 dpi鉴定了许多解除管制的途径和基因家族。“植物-病原体互作”途径与KEGG No。KO04626的nbs - lrrfamily基因在5 dpi时高度富集，数十个nbs - lrrfamily基因显著下调。qRT-PCR分析验证了这些基因的表达模式，大多数与RNA-seq数据一致。由于NBS-LRR家族蛋白广泛参与植物免疫应答，我们的研究结果表明，这个时间点（5 dpi）对smv -大豆相互作用很重要。与此一致的是，SMV滴度从1 dpi增加到10 dpi，并在5 dpi时达到峰值。SMV感染诱导SA（水杨酸）标记基因pr -1的表达。外源MeSA （SA的一种活性形式）的施用可以增强大豆对病毒感染的抗性，减少SMV在大豆中的积累。有趣的是，MeSA治疗也显著上调了SMV-responsiveNBS-LRRgenes的表达。抗性品种RV8143受SMV感染后，内源SA水平高于感病品系和风25，且与感病品系一致。此外，nbs - lrrfamily基因在RV8143感染SMV后表达上调，而在合丰25感染SMV后表达下调。我们的结果表明，SA和nbs - lrrfamily基因参与了smv -大豆相互作用。SMV可能通过抑制nbs - lrrfamily基因而影响大豆的防御反应，SA参与了这一相互作用过程。
Soybean mosaic virus(SMV) is a severe pathogen reducing crop yield and seed quality of soybean. Although several resistance gene loci includingRsv1,Rsv3 andRsv4 are identified in some soybean varieties, most of the soybean genes related to SMV infection are still not characterized. In order to reveal genome-wide gene expression profiles in response to SMV infection, we used transcriptome analysis to determine SMV-responsive genes in susceptible variety Hefeng25. Time course RNA-seq analysis at 1, 5 and 10 dpi identified many deregulated pathways and gene families. “Plant-pathogen interaction” pathway with KEGG No. of KO04626 was highly enriched and dozens ofNBS-LRRfamily genes were significantly down-regulated at 5 dpi. qRT-PCR analyses were performed to verify expression patterns of these genes and most were in accordance with the RNA-seq data. As NBS-LRR family proteins are broadly involved in plant immunity responses, our results indicated the importance of this time point (5 dpi) for SMV-soybean interaction. Consistent with it, SMV titer was increased from 1 dpi to 10 dpi and peaked at 5 dpi. Expression of SA (salicylic acid) marker genePR-1was induced by SMV infection. Application of exogenous MeSA, an active form of SA, primed the plant resistant to virus infection and reduced SMV accumulation in soybean. Interestingly, MeSA treatment also significantly upregulated expressions of SMV-responsiveNBS-LRRgenes. Compared with susceptible line Hefeng25, endogenous SA level was higher and was consistently induced by SMV infection in resistant variety RV8143. Moreover, expressions ofNBS-LRRfamily genes were up-regulated by SMV infection in RV8143, while they were down-regulated by SMV infection in Hefeng25. Our results implied that SA andNBS-LRRfamily genes were involved in SMV-soybean interaction. SMV could compromise soybean defense responses by repression ofNBS-LRRfamily genes in Hefeng25, and SA was implicated in this interaction process.