A new ultrasound instrument for in vivo microimaging of mice
A new ultrasound instrument for in vivo microimaging of mice
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DOI：
10.1016/s0301-5629(02)00567-7
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发表时间：
2002-09-01
影响因子：
2.9
通讯作者：
Adamson, SL
中科院分区：
文献类型：
--
作者：
Foster, FS;Zhang, MY;Adamson, SL
我们在此报告第一个专门用于小鼠显微成像的高频超声 (US) 成像系统的设计和评估。高频超声或超声生物显微镜（UBM）具有成本低、成像速度快、便携和高分辨率的优点。结合提供血流功能信息的能力，UBM 为研究发育和疾病模型提供了一种强大的方法。新的 UBM 成像系统适用于从胚胎发生第 5.5 天到成年小鼠的小鼠发育。在 40 MHz 频率下，新型鼠标扫描仪的体素分辨率为 57 mum x 57 mum x 40 mum。双工多普勒提供毫米/秒范围内的血流速度灵敏度，与微循环中的血流一致，并且可以轻松检测胚胎小鼠心脏、主动脉、肝脏和胎盘中的血流量。无创 UBM 发育评估与小鼠解剖学的侵入性图谱惊人相似。介绍了使用任何成像方法获得的最详细的小鼠胚胎发育的无创体内图像。 (C) 2002 年世界超声医学生物学联合会。
We report here on the design and evaluation of the first high-frequency ultrasound (US) imaging system specifically designed for microimaging of the mouse. High-frequency US or US biomicroscopy (UBM) has the advantage of low cost, rapid imaging speed, portability and high resolution. In combination with the ability to provide functional information on blood flow, UBM provides a powerful method for the investigation of development and disease models. The new UBM imaging system is demonstrated for mouse development from day 5.5 of embryogenesis through to the adult mouse. At a frequency of 40 MHz, the resolution voxel of the new mouse scanner measures 57 mum x 57 mum x 40 mum. Duplex Doppler provides blood velocity sensitivity to the mm per s range, consistent with flow in the microcirculation, and can readily detect blood How in the embryonic mouse heart, aorta, liver and placenta. Noninvasive UBM assessment of development shows striking similarity to invasive atlases of mouse anatomy. The most detailed noninvasive in vivo images of mouse embryonic development achieved using any imaging method are presented. (C) 2002 World Federation for Ultrasound in Medicine Biology.