Cohesin cleavage by separase required for anaphase and cytokinesis in human cells
Cohesin cleavage by separase required for anaphase and cytokinesis in human cells
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DOI：
10.1126/science.1061376
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发表时间：
2001-08-17
期刊：
影响因子：
56.9
通讯作者：
Peters, JM
中科院分区：
文献类型：
--
作者：
Hauf, S;Waizenegger, IC;Peters, JM
细胞分裂依赖于后期姐妹染色单体的分离。在酵母中，姐妹分离是由蛋白酶分离酶对粘附素的裂解而开始的。在脊椎动物中，大多数粘附素是通过一种不依赖于切割的机制从染色体臂上移除的。只有残留量的粘附素在着丝粒后期开始被切割，这与它从着丝粒中消失的时间一致。我们已经在人类粘附素亚基SCC1中鉴定了两个分离酶切割位点，并在人类细胞中有条件地表达了不可切割的SCC1突变体。我们的结果表明，分离酶对粘附素的切割对于姐妹染色单体的分离和细胞质分裂的完成是必不可少的。
Cell division depends on the separation of sister chromatids in anaphase. In yeast, sister separation is initiated by cleavage of cohesin by the protease separase. In vertebrates, most cohesin is removed from chromosome arms by a cleavage-independent mechanism. Only residual amounts of cohesin are cleaved at the onset of anaphase, coinciding with its disappearance from centromeres. We have identified two separase cleavage sites in the human cohesin subunit SCC1 and have conditionally expressed noncleavable SCC1 mutants in human cells. our results indicate that cohesin cleavage by separase is essential for sister chromatid separation and for the completion of cytokinesis.