Granulocyte colony-stimulating factor receptors on human acute leukemia: biphenotypic leukemic cells possess granulocyte colony-stimulating factor receptors.
Granulocyte colony-stimulating factor receptors on human acute leukemia: biphenotypic leukemic cells possess granulocyte colony-stimulating factor receptors.
复制标题
DOI：
--
复制
发表时间：
1992-06
期刊：
影响因子：
11.2
通讯作者：
K. Shimoda;S. Okamura;N. Harada;Wataru Ikematsu;S. Kondo;C. Kawasaki;Takeshi I Anaka;Tetsuya Etou;Kouichi Akashi;Takashi Okamura;Tsunefumi Shibuya;Mine Harada;Yoshiyuki Niho;Y. Nj
中科院分区：
文献类型：
--
作者：
K. Shimoda;S. Okamura;N. Harada;Wataru Ikematsu;S. Kondo;C. Kawasaki;Takeshi I Anaka;Tetsuya Etou;Kouichi Akashi;Takashi Okamura;Tsunefumi Shibuya;Mine Harada;Yoshiyuki Niho;Y. Nj
用流式细胞术检测新诊断的急性白血病或慢性骨髓性白血病危象患者门控白血病母细胞上的粒细胞集落刺激因子（G-CSF）受体。表面标记分析和细胞化学研究同时进行，以表征胚细胞。在24例白血病病例中，尽管阳性细胞的百分比范围差异很大，但在所有11例急性髓母细胞白血病中均检测到G-CSF受体阳性的母细胞。另一方面，在无骨髓表面抗原的过氧化物酶阴性急性淋巴细胞白血病患者的胚细胞中未检测到它们。然而，G-CSF受体在8例表达骨髓和淋巴表面抗原的过氧化物酶阴性急性白血病（双表型白血病）中5例的母细胞中被证实有显著量。双表型组G-CSF受体阳性的母细胞百分比[33 +/- 14% (SD)]明显低于急性髓母细胞白血病组[65 +/- 22%]（P < 0.01）。胚细胞CD13抗原表达率与G-CSF受体阳性表达率呈显著相关（rs = 0.50, P < 0.05）。这些发现表明，流式细胞术检测到的G-CSF受体在具有髓系特征的白血病细胞上的分布可能与成熟过程有关。
Granulocyte colony-stimulating factor (G-CSF) receptors on the gated leukemic blast cells from newly diagnosed patients with acute leukemia or crisis of chronic myelogenous leukemia were investigated using flow cytometric detection. Surface marker analysis and cytochemical studies were conducted simultaneously to characterize the blast cells. Among 24 leukemia cases examined, G-CSF receptor-positive blast cells were detected in all 11 cases of acute myeloblastic leukemia even though the percentage range of positive cells was widely variable. On the other hand, they were not detected on the blast cells from patients with peroxidase-negative acute lymphoblastic leukemia with no myeloid surface antigens. However, G-CSF receptors were demonstrated in significant amounts on blast cells from 5 of 8 cases of peroxidase-negative acute leukemia expressing both myeloid and lymphoid surface antigens (biphenotypic leukemia). The percentage of blast cells positive for G-CSF receptors was significantly smaller in biphenotypic cases [33 +/- 14% (SD)] than in acute myeloblastic leukemia cases [65 +/- 22%] (P less than 0.01). The percentage expression of CD13 antigen by blast cells was significantly related to their percentage positivity for G-CSF receptors (rs = 0.50, P less than 0.05). These findings indicate that the distribution of flow cytometrically detectable G-CSF receptors on leukemic cells possessing myeloid characteristics may be related to the maturation process.