The chimeric repressor version of an Ethylene Response Factor (ERF) family member, Sl-ERF.B3, shows contrasting effects on tomato fruit ripening
The chimeric repressor version of an Ethylene Response Factor (ERF) family member, Sl-ERF.B3, shows contrasting effects on tomato fruit ripening
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DOI：
10.1111/nph.12771
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发表时间：
2014-07-01
期刊：
影响因子：
9.4
通讯作者：
Bouzayen, Mondher
中科院分区：
文献类型：
--
作者：
Liu, Mingchun;Diretto, Gianfranco;Bouzayen, Mondher
果实成熟涉及乙烯和成熟相关转录调节因子之间复杂的相互作用。乙烯反应因子（ERF）是乙烯信号的下游组分，已知其调节乙烯反应基因的表达。ERF.B3-SRDX基因在番茄（Solanum lycopersicum）果实成熟过程中的作用研究表明，ERF.B3-SRDX基因的过量表达可显著延缓果实成熟，提高跃变型乙烯的产生，促进果实软化，减少色素的积累。与此相一致的是，参与乙烯生物合成和软化的基因被上调，而类胡萝卜素生物合成的基因被下调。此外，在ERF. B3-SRDX显性阻遏物果实中，RIN、NOR、CNR和HB-1等成熟调节因子的表达受到刺激，许多ERF基因的表达模式发生了严重改变，表明ERF基因之间存在一个复杂的网络，这可能是果实成熟和成熟过程中多效性改变的原因。总的来说，该研究揭示了S1-ERF.B3在控制成熟过程的转录网络中的作用，并揭示了解偶联一些主要成熟相关过程的手段。
Fruit ripening involves a complex interplay between ethylene and ripening-associated transcriptional regulators. Ethylene Response Factors (ERFs) are downstream components of ethylene signaling, known to regulate the expression of ethylene-responsive genes. Although fruit ripening is an ethylene-regulated process, the role of ERFs remains poorly understood.The role of Sl-ERF.B3 in tomato (Solanum lycopersicum) fruit maturation and ripening is addressed here using a chimeric dominant repressor version (ERF.B3-SRDX).Over-expression of ERF.B3-SRDX results in a dramatic delay of the onset of ripening, enhanced climacteric ethylene production and fruit softening, and reduced pigment accumulation. Consistently, genes involved in ethylene biosynthesis and in softening are up-regulated and those of carotenoid biosynthesis are down-regulated. Moreover, the expression of ripening regulators, such as RIN, NOR, CNR and HB-1, is stimulated in ERF.B3-SRDX dominant repressor fruits and the expression pattern of a number of ERFs is severely altered.The data suggest the existence of a complex network enabling interconnection between ERF genes which may account for the pleiotropic alterations in fruit maturation and ripening. Overall, the study sheds new light on the role of Sl-ERF.B3 in the transcriptional network controlling the ripening process and uncovers a means towards uncoupling some of the main ripening-associated processes.