Circulating microRNAs and adipokines as markers of metabolic syndrome in adolescents with obesity
Circulating microRNAs and adipokines as markers of metabolic syndrome in adolescents with obesity
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DOI：
10.1016/j.clnu.2018.09.024
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发表时间：
2019-10-01
影响因子：
6.3
通讯作者：
Al-Rawaf, Hadeel A.
中科院分区：
文献类型：
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作者：
Al-Rawaf, Hadeel A.
背景资料：循环microRNA（microRNAs，miRNAs）是研究肥胖症发病机制的重要生物标志物。目的：探讨肥胖症患者循环microRNA表达谱及其与瘦素和脂联素水平的关系。方法：采用RT-PCR和免疫组化方法研究肥胖症患者循环microRNA表达谱、脂联素和瘦素水平。结果：在病态肥胖青少年中，我们发现了至少10种循环miRNAs，包括miRNAs浓度升高; miR-142- 3 p、miR-140- 5 p、miR-222、miR-143、miR-130以及miR-532- 5 p、miR-423- 5 p、miR-520 c-3 p、miR-146 a和miR-15 a浓度降低，这与BMI、WHtR、脂肪因子的测量值密切相关;脂联素、瘦素、L/A比值和其他MS相关生物标志物如FBS、胰岛素、HOMA-IR、C肽以及循环血脂如TG、HDL-C和LDL-C。重度肥胖青少年的脂联素、瘦素和L/A比值该研究提供了miRNA表达的调节与脂肪因子和其他相关的MS代谢因子相关。因此，早期检测循环miRNA谱的任何变化可能在识别可能患有严重代谢综合征的肥胖儿童或青少年中发挥有希望的作用。(C)2018爱思唯尔有限公司和欧洲临床营养与代谢学会。All rights reserved.
Background: Circulating microRNAs (miRNAs) as valuable biomarkers yielded important insights into the pathogenesis of obesity.Aim: This study aimed to describe the circulating miRNA profile for adolescences and its association with the circulating levels leptin and adiponectin according to specific degree of obesity.Methods: RT-PCR and immunoassy analysis were used to study circulating miRNA profile, adipokines; adiponectin (A), leptin (L), and L/A ratio as well as other factors of metabolic syndrome (MS) in 250 adolescents with severe obesity.Results: In morbidly obese adolescents, we identified at least 10 circulating miRNAs, including increased concentrations of miRNAS; miR-142-3p, miR-140-5p, miR-222 miR-143, miR-130, and decreased concentrations of miR-532-5p, miR-423-5p, miR-520c-3p, miR-146a, and miR-15a, which were strongly linked to measures of BMI, WHtR, adipokines; adiponectin, leptin, L/A ratio, and other MS related biomarkers such as FBS, insulin, HOMA-IR, C-peptide, and circulated plasma lipids such as TG, HDL-C, and LDL-C.Conclusion: Circulating miRNAs showed significant association with plasma levels of adipokines; adiponectin, leptin, and L/A ratios in adolescents with severe obesity. The study provides that regulation of miRNAs expression is associated with adipokines, and other related MS metabolic factors. Thus, early detection of any changes in circulating miRNAs profiles may play a promising role in identifying obese children or adolescents who may suffer from severe metabolic syndrome. (C) 2018 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.