Baculovirus surface display of Erns envelope glycoprotein of classical swine fever virus
Baculovirus surface display of Erns envelope glycoprotein of classical swine fever virus
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DOI：
10.1016/j.jviromet.2008.07.019
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发表时间：
2008-11-01
影响因子：
3.1
通讯作者：
Liu, Hung-Jen
中科院分区：
文献类型：
--
作者：
Xu, Xin-Gang;Chiou, Ming-Tang;Liu, Hung-Jen
猪瘟病毒（CSFV）在许多国家的养猪业中造成重大损失。Ell是CSFV的包膜糖蛋白，已知其在天然宿主中诱导病毒中和抗体和保护性免疫。本研究构建了一种表达杆状病毒囊膜蛋白gp 64的跨膜区（TM）和胞浆区（CTD）的重组杆状病毒BacSC-E-rns，并在小鼠模型中评价了其免疫效果。Western blot和共聚焦显微镜观察显示，感染后，Em在Sf-9细胞中表达并锚定在细胞膜上。免疫金电镜显示，杆状病毒包膜上成功地展示了Ell糖蛋白。动物疫苗试验表明，BacSC-E-rns免疫小鼠后，E-rns抗体滴度明显高于对照组。这表明BacSC-E-rns疫苗可以潜在地用于对抗CSFV感染。这是第一份证明E-rns-假型杆状病毒作为CSFV疫苗的潜力的报告。(C)2008 Elsevier B. V.保留所有权利。
Classical swine fever virus (CSFV) causes significant losses in the pig industry in many countries. Ell is an envelope glycoprotein of CSFV which is known to induce virus-neutralizing antibodies and protective immunity in the natural host. In this study, one recombinant baculoviruses BacSC-E-rns expressing histidine-tagged Ell with the transmembrane domain (TM) and cytoplasmic domain (CTD) derived from baculovirus envelope protein gp64 was constructed and its immunizing efficacy was evaluated in a mouse model. After infection, Em, was expressed and anchored on the plasma membrane of Sf-9 cells, as demonstrated by Western-blot and confocal microscopy. Immunogold electron microscopy demonstrated that the Ell glycoprotein was successfully displayed on the baculoviral envelope. Vaccine tests in animals showed that BacSC-E-rns elicited significantly higher E-rns antibody titers in the immunized mouse models than the control group. This demonstrates that the BacSC-E-rns vaccine can be used potentially against CSFV infections. This is the first report demonstrating the potential of E-rns-pseudotyped baculovirus as a CSFV vaccine. (C) 2008 Elsevier B.V. All rights reserved.