Molecular analysis of T cell receptor (Ti) variable region (V) gene expression. Evidence that a single Ti beta V gene family can be used in formation of V domains on phenotypically and functionally diverse T cell populations.
Molecular analysis of T cell receptor (Ti) variable region (V) gene expression. Evidence that a single Ti beta V gene family can be used in formation of V domains on phenotypically and functionally diverse T cell populations.
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DOI：
10.1084/jem.161.6.1326
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发表时间：
1985-06-01
期刊：
影响因子：
--
通讯作者：
Reinherz EL
中科院分区：
文献类型：
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作者：
Acuto O;Campen TJ;Royer HD;Hussey RE;Poole CB;Reinherz EL
我们研究了在不同的调节和效应T淋巴细胞亚群中T细胞抗原-MHC受体形成中Ti β可变（V）基因片段使用的规则。为此，分析了单个Ti β V基因家族及其产物。一种被称为抗Ti 3A的单克隆抗体被用于从未引发的外周T淋巴细胞中选择克隆，该单克隆抗体显示出与雷克斯细胞系Ti β V基因家族成员编码的表位反应，并且在2%的人T淋巴细胞上表达。定义了具有诱导、抑制和/或细胞毒性功能的T4+和T8+ T细胞克隆。Southern分析、等电聚焦和二维肽图谱表明雷克斯V基因家族的各个成员与不同的Ti β多样性和/或连接和恒定区片段相关联。此外，这些克隆的Ti α链是不同的。这些结果意味着Ti β V基因的使用不限于任何功能或表型定义的T细胞亚群，并且可能对产生组合、连接或链缔合介导的多样性的机制几乎没有限制。
We examine the rules governing Ti beta variable (V) gene segment usage in the formation of T cell antigen-MHC receptors in diverse regulatory and effector T lymphoid subpopulations. To this end, a single Ti beta V gene family and its products were analyzed. A monoclonal antibody, termed anti-Ti3A, which was shown to be reactive with an epitope encoded by members of the REX cell line Ti beta V gene family, and which was expressed on 2% of human T lymphocytes was used in selection of clones from unprimed peripheral T lymphocytes. Both T4+, as well as T8+ T cell clones with inducer, suppressor, and/or cytotoxic function were defined. Southern analysis, isoelectric focusing and two- dimensional peptide mapping indicated that individual members of the REX V gene family were linked to different Ti beta diversity and/or joining and constant region segments. Moreover, the Ti alpha chains of such clones were distinct. These results imply that Ti beta V gene usage is not restricted to any functionally or phenotypically defined T cell subsets, and there is presumably little, if any, restriction on the mechanisms that generate combinational, junctional or chain association-mediated diversity.