Bacterial community structures in honeybee intestines and their response to two insecticidal proteins
Bacterial community structures in honeybee intestines and their response to two insecticidal proteins
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DOI：
10.1111/j.1574-6941.2006.00249.x
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发表时间：
2007-03-01
影响因子：
4.2
通讯作者：
Widmer, Franco
中科院分区：
文献类型：
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作者：
Babendreier, Dirk;Joller, David;Widmer, Franco
在本研究中，研究了 Bt 毒素 Cry1Ab 和大豆胰蛋白酶抑制剂 (SBTI) 对成年蜜蜂 (Apis mellifera) 肠道细菌群落的影响。据推测，蜜蜂肠道细菌群落的变化可能代表肠道生理学改变的敏感指标。在实验室装置中，用 Bt 转基因品种 MON810 或非转基因近亲缘植物的玉米花粉喂养蜜蜂。纯化的 Cry1Ab (0.0014% w/v) 和 SBTI (0.1% 或 1% w/v) 代表补充治疗。为了进行比较，我们对瑞士两个地点的自由飞行蜜蜂进行了分析。细菌 16S rRNA 基因片段的 PCR 扩增和末端限制性片段长度多态性分析显示总共 17 个不同的末端限制性片段 (T-RF)，这些片段在实验室饲养的蜜蜂和自由飞行的蜜蜂之间高度一致。 T-RF 隶属于 Alpha、Beta 和 Gammaproteobacteria、厚壁菌门和拟杆菌门。 Bt 玉米花粉和高浓度的 Cry1Ab 都没有显着影响蜜蜂肠道中的细菌群落。只有高浓度的 SBTI 才能显着减少蜜蜂中肠中检测到的 T-RF 数量，该浓度也会增加蜜蜂死亡率。因此，总细菌群落结构可能不是为杀虫蛋白在亚致死水平对蜜蜂的影响提供证据的敏感指标。
In this study, the effects of the Bt-toxin Cry1Ab and a soybean trypsin inhibitor (SBTI) on intestinal bacterial communities of adult honeybees (Apis mellifera) were investigated. It was hypothesized that changes in intestinal bacterial communities of honeybees may represent a sensitive indicator for altered intestinal physiology. Honeybees were fed in a laboratory set-up with maize pollen from the Bt-transgenic cultivar MON810 or from the non-transgenic near isoline. Purified Cry1Ab (0.0014% w/v) and SBTI (0.1% or 1% w/v) represented supplementary treatments. For comparison, free-flying honeybees from two locations in Switzerland were analysed. PCR-amplification of bacterial 16S rRNA gene fragments and terminal restriction fragment length polymorphism analyses revealed a total of 17 distinct terminal restriction fragments (T-RFs), which were highly consistent between laboratory-reared and free-flying honeybees. The T-RFs were affiliated to Alpha-, Beta-, and Gammaproteobacteria, to Firmicutes, and to Bacteriodetes. Neither Bt-maize pollen nor high concentrations of Cry1Ab significantly affected bacterial communities in honeybee intestines. Only the high concentration of SBTI significantly reduced the number of T-RFs detected in honeybee midguts, a concentration that also increases bee mortality. Therefore, total bacterial community structures may not be a sensitive indicator for providing evidence for the impact of insecticidal proteins on honeybees at sublethal levels.