Paralytic Shellfish Toxin Uptake, Assimilation, Depuration, and Transformation in the Southeast Asian Green-Lipped Mussel (Perna viridis)
Paralytic Shellfish Toxin Uptake, Assimilation, Depuration, and Transformation in the Southeast Asian Green-Lipped Mussel (Perna viridis)
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DOI：
10.3390/toxins11080468
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发表时间：
2019-08-01
期刊：
影响因子：
4.2
通讯作者：
Azanza, Rhodora V.
中科院分区：
文献类型：
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作者：
Andres, John Kristoffer;Yniguez, Aletta T.;Azanza, Rhodora V.
双壳类软体动物是菲律宾重要的食物来源，但食用贝类组织中有害藻类毒素的积累损害了海产品消费者的健康。为了评估贝类毒素风险的动态，本研究研究了麻痹贝类毒素在绿滨螺（Perna viridis）体内的吸收、净化、同化和类似物变化。对贻贝进行了水箱实验，用有毒的甲藻亚历山大菌喂食。在六天的时间里对水和贝类进行采样，以确定贝类肉和水中的毒素浓度以及藻类细胞密度。测定的最大总毒素浓度为367 μ g STX当量/100 g贝类组织，比菲律宾监管行动限值高出6倍多。在研究期间观察到几个摄取和净化周期，第一个在前24小时内观察到的周期与高藻类细胞密度相吻合。在贝类组织的不同部位评估了毒素负荷，在前18小时内，地幔中的毒素水平最高，但随后向肠道转移。毒素谱数据的比较证明了源藻类中的gtx1,4转化为贝类组织中效力较弱的gtx2,3。总的来说，该研究说明了在模拟藻华事件期间，绿藻毒素浓度的时间变化，以及即使在有毒藻类被清除后，水中毒素的积累。
Bivalve molluscs represent an important food source within the Philippines, but the health of seafood consumers is compromised through the accumulation of harmful algal toxins in edible shellfish tissues. In order to assess the dynamics of toxin risk in shellfish, this study investigated the uptake, depuration, assimilation, and analogue changes of paralytic shellfish toxins in Perna viridis. Tank experiments were conducted where mussels were fed with the toxic dinoflagellate Alexandrium minutum. Water and shellfish were sampled over a six day period to determine toxin concentrations in the shellfish meat and water, as well as algal cell densities. The maximum summed toxin concentration determined was 367 mu g STX eq./100 g shellfish tissue, more than six times higher than the regulatory action limit in the Philippines. Several uptake and depuration cycles were observed during the study, with the first observed within the first 24 h coinciding with high algal cell densities. Toxin burdens were assessed within different parts of the shellfish tissue, with the highest levels quantified in the mantle during the first 18 h period but shifting towards the gut thereafter. A comparison of toxin profile data evidenced the conversion of GTX1,4 in the source algae to the less potent GTX2,3 in the shellfish tissue. Overall, the study illustrated the temporal variability in Perna viridis toxin concentrations during a modelled algal bloom event, and the accumulation of toxin from the water even after toxic algae were removed.