Tandem mass tags:: A novel quantification strategy for comparative analysis of complex protein mixtures by MS/MS
Tandem mass tags:: A novel quantification strategy for comparative analysis of complex protein mixtures by MS/MS
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DOI：
10.1021/ac0262560
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发表时间：
2003-04-15
影响因子：
7.4
通讯作者：
Hamon, C
中科院分区：
文献类型：
--
作者：
Thompson, A;Schäfer, J;Hamon, C
描述了一种新的基于MS/MS的分析策略，其使用同位素异构体标记，称为“串联质量标签”（TMT），用于肽和蛋白质的准确定量。新标签的设计旨在确保用不同TMT标记的相同肽在所有分离中准确共迁移。标签需要使用串联质谱的定量分析的新方法。新的标签和分析方法允许来自不同样品的肽通过其相对丰度进行鉴定，比其他方法更容易和准确。新的TMT允许使用基于碰撞诱导解离（CID）的分析方法同时测定肽对的身份和相对丰度。在MS/MS模式下使用新标签进行的相对丰度测量准确且灵敏。与MS模式测量相比，基于MS/MS的检测实现了非常高的信噪比。新的标签应该适用于各种各样的肽分离方法。
A novel MS/MS-based analysis strategy using isotopomer labels, referred to as "tandem mass tags" (TMTs), for the accurate quantification of peptides and proteins is described. The new tags are designed to ensure that identical peptides labeled with different TMTs exactly comigrate in all separations. The tags require novel methods of quantification analysis using tandem mass spectrometry. The new tags and analysis methods allow peptides from different samples to be identified by their relative abundance with greater ease and accuracy than other methods. The new TMTs permit simultaneous determination of both the identity and relative abundances of peptide pairs using a collision induced dissociation (CID)-based analysis method. Relative abundance measurements made in the MS/MS mode using the new tags are accurate and sensitive. Compared to MS-mode measurements, a very high signal-to-noise ratio is achieved with MS/MS based detection. The new tags should be applicable to a wide variety of peptide isolation methods.