Arabidopsis Class I and Class II TCP Transcription Factors Regulate Jasmonic Acid Metabolism and Leaf Development Antagonistically
Arabidopsis Class I and Class II TCP Transcription Factors Regulate Jasmonic Acid Metabolism and Leaf Development Antagonistically
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DOI：
10.1104/pp.112.200303
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发表时间：
2012-08-01
期刊：
影响因子：
7.4
通讯作者：
Immink, Richard G. H.
中科院分区：
文献类型：
--
作者：
Danisman, Selahattin;van der Wal, Froukje;Immink, Richard G. H.
TEOSINTE BRANCHED 1/Cycloidea/PROLIFERATING CELL FACTOR 1（TCP）转录因子控制植物的发育过程。在拟南芥（Arabidopsis thaliana）基因组中编码的24个TCP转录因子被分为两类，I类和II类TCP，它们被提出拮抗作用。我们进行了详细的表型分析的I类tcp 20突变体，在10天的老苗叶铺面细胞大小的增加。随后，进行糖皮质激素受体诱导测定，旨在鉴定叶片发育过程中TCP 20蛋白的潜在靶基因。脂氧合酶2（LOX 2）和I类TCP 9基因被鉴定为TCP 20靶标，TCP 20与其调控序列的结合可通过染色质免疫沉淀分析证实。LOX 2编码茉莉酸生物合成基因，其也被II类TCP蛋白靶向，所述II类TCP蛋白在微小RNA JAGGED AND WAVY（JAW）的控制下，尽管是以拮抗方式。TCP 9的突变，第二个确定的TCP 20目标，导致在早期叶发育阶段的路面细胞的大小增加。在单一的tcp 9和tcp 20突变体和tcp 9 tcp 20双突变体衰老的分析表明，这一过程的早期发作相比，野生型对照植物中的双突变体。细胞大小和衰老表型都与JAW植物中已知的II类TCP突变体表型相反。总之，这些结果指出，拮抗功能的I类和II类TCP蛋白在控制叶发育通过茉莉酸信号通路。
TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR1 (TCP) transcription factors control developmental processes in plants. The 24 TCP transcription factors encoded in the Arabidopsis (Arabidopsis thaliana) genome are divided into two classes, class I and class II TCPs, which are proposed to act antagonistically. We performed a detailed phenotypic analysis of the class I tcp20 mutant, showing an increase in leaf pavement cell sizes in 10-d-old seedlings. Subsequently, a glucocorticoid receptor induction assay was performed, aiming to identify potential target genes of the TCP20 protein during leaf development. The LIPOXYGENASE2 (LOX2) and class I TCP9 genes were identified as TCP20 targets, and binding of TCP20 to their regulatory sequences could be confirmed by chromatin immunoprecipitation analyses. LOX2 encodes for a jasmonate biosynthesis gene, which is also targeted by class II TCP proteins that are under the control of the microRNA JAGGED AND WAVY (JAW), although in an antagonistic manner. Mutation of TCP9, the second identified TCP20 target, resulted in increased pavement cell sizes during early leaf developmental stages. Analysis of senescence in the single tcp9 and tcp20 mutants and the tcp9tcp20 double mutants showed an earlier onset of this process in comparison with wild-type control plants in the double mutant only. Both the cell size and senescence phenotypes are opposite to the known class II TCP mutant phenotype in JAW plants. Altogether, these results point to an antagonistic function of class I and class II TCP proteins in the control of leaf development via the jasmonate signaling pathway.