Effect of anti-LFA-1 monoclonal antibody on rat small bowel allograft survival and circulating leukocyte populations
Effect of anti-LFA-1 monoclonal antibody on rat small bowel allograft survival and circulating leukocyte populations
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DOI：
10.1016/s0966-3274(00)00007-1
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发表时间：
2000-03-01
影响因子：
1.5
通讯作者：
Wood, RFM
中科院分区：
文献类型：
--
作者：
Bowles, MJ;Pockley, AG;Wood, RFM
在几种动物模型中，抗LFA-L的单抗可延长移植物存活时间。本研究评价了抗LFA-L单抗对同种异体小肠移植排斥反应、循环白细胞亚群和体内靶细胞抗原阻断的影响。以PVG供体大鼠和DA受体大鼠为供体进行异位小肠移植。移植动物分别于移植后第1、0天和第1天给予WT1 1 mg/(kg·d)灌胃。另一组未移植动物同时给予WT1 3个剂量，以监测循环白细胞数量和体内结合情况。WT1使受者存活时间从7天延长至14天。外周血白细胞数增加了两倍多，主要是由于CD4(+)和CD8(+)淋巴细胞的显著增加。治疗结束后，淋巴细胞和单核细胞上约85%的WT1结合位点被阻断/调节。WT1对循环白细胞和靶细胞结合能力有明显影响，并可影响大鼠小肠移植受体的存活。(C)2000 Elsevier Science B.V.保留所有权利。
Anti-LFA-l monoclonal antibodies (mAb) prolong graft survival in several animal models. This study assessed the effect of an anti-LFA-l mAb (WT.1) on small bowel allograft rejection, circulating leukocyte subsets and in vivo target cell antigen blockade. Heterotopic small bowel transplantation was performed between PVG donor and DA recipient rats. Transplanted animals received 1 mg/kg per day WT.1 on days - 1, 0 (day of transplantation) and 1. Three doses of WT.1 were also administered to a group of untransplanted animals to monitor circulating leukocyte populations and in vivo binding. WT.1 prolonged recipient survival from 7 to 14 days. Peripheral leukocyte counts increased more than twofold, primarily due to marked increases in both CD4(+) and CD8(+) lymphocytes. Approximately 85% of WT.1 binding sites on lymphocytes and monocytes were blocked/modulated after the course of therapy. WT.1 has marked effects on circulating leukocytes and target cell binding capacities and can affect the survival of rat small bowel transplant recipients. (C) 2000 Elsevier Science B.V. All rights reserved.