Analysis of Precursors to Wine Odorant 3-Mercaptohexan-1-ol Using HPLC-MS/MS: Resolution and Quantitation of Diastereomers of 3-S-Cysteinylhexan-1-ol and 3-S-Glutathionylhexan-1-ol
Analysis of Precursors to Wine Odorant 3-Mercaptohexan-1-ol Using HPLC-MS/MS: Resolution and Quantitation of Diastereomers of 3-S-Cysteinylhexan-1-ol and 3-S-Glutathionylhexan-1-ol
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DOI：
10.1021/jf903720w
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发表时间：
2010-02-10
影响因子：
6.1
通讯作者：
Jeffery, David W.
中科院分区：
文献类型：
--
作者：
Capone, Dimitra L.;Sefton, Mark A.;Jeffery, David W.
建立了一种分析葡萄汁和葡萄酒中3-S-半胱氨酰己-1-醇（Cys-3-MH）和3-S-谷胱甘肽己-1-醇（Glut-3-MH）非对映异构体的方法。该方法采用稳定同位素稀释分析（SIDA）结合固相萃取（SPE）和反相高效液相色谱-串联质谱（HPLC-MS/MS）进行定量。这些化合物被认为是重要的葡萄酒增香剂3-巯基己-1-醇（3-MH）的潜在前体。这构成了第一种分析方法，其中（1）葡萄汁和葡萄酒中的Glut-3-MH已被准确定量，（2）单个Cys-和Glut-3-MH非对映异构体通过单一HPLC-MS/MS方法分离和定量。氘标记内标物的使用已导致准确和精密的方法，可达到定量限
A method has been developed and validated for the analysis of the individual diastereomers of 3-S-cysteinylhexan-1-ol (Cys-3-MH) and 3-S-glutathionylhexan-1-ol (Glut-3-MH) extracted from grape juice and wine. The method uses stable isotope dilution analysis (SIDA) combined with solid-phase extraction (SPE) and reversed-phase high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) for quantitation. These compounds have been considered as potential precursors to the important wine odorant 3-mercaptohexan-1-ol (3-MH). This constitutes the first analytical method where (1) Glut-3-MH has been accurately quantified in grape juice and wine and (2) the individual Cys- and Glut-3-MH diastereomers were separated and quantified by a single HPLC-MS/MS method. The use of deuterium-labeled internal standards has resulted in an accurate and precise method that can achieve quantitation limits of