ARABIDOPSIS-THALIANA NADPH OXIDOREDUCTASE HOMOLOGS CONFER TOLERANCE OF YEASTS TOWARD THE THIOL-OXIDIZING DRUG DIAMIDE
ARABIDOPSIS-THALIANA NADPH OXIDOREDUCTASE HOMOLOGS CONFER TOLERANCE OF YEASTS TOWARD THE THIOL-OXIDIZING DRUG DIAMIDE
复制标题
DOI：
10.1074/jbc.270.44.26224
复制
发表时间：
1995-11-03
影响因子：
4.8
通讯作者：
INZE, D
中科院分区：
文献类型：
--
作者：
BABIYCHUK, E;KUSHNIR, S;INZE, D
为了分离新的植物基因参与防御氧化应激，拟南芥cDNA文库在酵母表达载体转化到酵母菌株缺陷的YAP 1基因，其编码的b-Zip转录因子和调节一般的压力反应在酵母。对来自约10（5）个原代转化体的细胞进行针对巯基氧化药物二酰胺的耐受性筛选，所述巯基氧化药物二酰胺消耗细胞中的还原型谷胱甘肽。分离出四种类型的拟拟南芥cDNA，这些cDNA中的三种（P1、Pa和P4）属于植物ζ-晶体蛋白家族，并且P3是拟南芥还原酶的同源物。因此，所有四种分离的cDNA都与NADPH氧化还原酶同源。PI，P2和PS稳态mRNA积累迅速在拟南芥植物在各种氧化应激条件下，如百草枯，叔丁基过氧化氢，二酰胺，甲萘醌处理。这些结果表明，这些cDNA编码的蛋白质在植物抗氧化防御中起着独特的作用，可能与NAD（P）/NAD（P）H的稳态有关。
To isolate new plant genes involved in the defense against oxidative stress, an Arabidopsis cDNA library in a yeast expression vector was transformed into a yeast strain deficient in the YAP1 gene, which encodes a b-Zip transcription factor and regulates general stress response in yeasts. Cells from approximate to 10(5) primary transformants were subjected to a tolerance screen toward the thiol-oxidizing drug diamide, which depletes the reduced glutathione in the cell, Four types of Arobidopsis cDNAs were isolated, Three of these cDNAs (PI, Pa, and P4) belong to a plant zeta-crystallin family and P3 is an Arabidopsis homolog of isoflavonoid reductases. As such, all four isolated cDNAs are homologous to NADPH oxidoreductases. PI, P2, and PS steady-state mRNAs accumulated rapidly in Arabidopsis plants under various oxidative stress conditions, such as treatment with paraquat, t-butylhydroperoxide, diamide, and menadione. The data suggested that proteins encoded by the isolated cDNAs play a distinct role in plant antioxidant defense and are possibly involved in NAD(P)/NAD(P)H homeostasis.