In vitro and in vivo antioxidant activity of exopolysaccharides from endophytic bacterium Paenibacillus polymyxa EJS-3
In vitro and in vivo antioxidant activity of exopolysaccharides from endophytic bacterium Paenibacillus polymyxa EJS-3
复制标题
内生细菌多粘类芽孢杆菌 EJS-3 胞外多糖的体外和体内抗氧化活性
DOI：
10.1016/j.carbpol.2010.07.008
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发表时间：
2010-11-11
影响因子：
11.2
通讯作者：
Zeng, Xiaoxiong
中科院分区：
文献类型：
--
作者：
Liu, Jun;Luo, Jianguang;Zeng, Xiaoxiong
通过体外和体内多种方法评估了内生细菌多粘类芽孢杆菌 EJS-3 的胞外多糖 (EPS) 的抗氧化活性。在体外抗氧化剂测定中，发现粗制 EPS 及其纯化组分（EPS-1 和 EPS-2）均具有中等的 2,2-二苯基-1-三硝基苯肼自由基清除活性、过氧化氢清除活性、脂质过氧化抑制作用和强亚铁离子螯合活性。 EPS体外抗氧化活性依次降低：粗EPS>EPS-2>EPS-1。在体内抗氧化剂测定中，小鼠皮下注射 D-半乳糖 (D-Gal) 6 周，同时通过灌胃施用 EPS-1。结果，给予EPS-1显着增加了D-Gal诱导的衰老小鼠的胸腺和脾脏指数。此外，EPS-1的施用显着增强了衰老小鼠的抗氧化酶活性和总抗氧化能力，并降低了血清和肝脏中丙二醛的水平。这些结果表明 EPS 具有有效的抗氧化活性，可以开发为新型天然抗氧化剂。 (C) 2010 Elsevier Ltd. 保留所有权利。
The antioxidant activities of exopolysaccharides (EPS) from endophytic bacterium Paenibacillus polymyxa EJS-3 were evaluated by various methods in vitro and in vivo. In antioxidant assays in vitro, both crude EPS and its purified fractions (EPS-1 and EPS-2) were found to have moderate 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity, hydrogen peroxide scavenging activity, lipid peroxidation inhibition effect, and strong ferrous ion chelating activity. And the antioxidant activities in vitro of EPS decreased in the order of crude EPS> EPS-2 > EPS-1. In antioxidant assays in vivo, mice were subcutaneously injected with D-galactose (D-Gal) for 6 weeks and administered EPS-1 via gavage simultaneously. As a result, administration of EPS-1 significantly increased the thymus and spleen indices of D-Gal induced aging mice. Moreover, EPS-1 administration significantly enhanced the activities of antioxidant enzymes and total antioxidant capacity and decreased the levels of malondialdehyde in both serums and livers of aging mice. These results suggested that EPS had potent antioxidant activities and could be explored as novel natural antioxidant. (C) 2010 Elsevier Ltd. All rights reserved.