Urodynamic findings in an awake chemical cystitis rat model observed by simultaneous registrations of intravesical and intraabdominal pressures.
Urodynamic findings in an awake chemical cystitis rat model observed by simultaneous registrations of intravesical and intraabdominal pressures.
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DOI：
10.5213/inj.2010.14.1.54
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发表时间：
2010-04-01
影响因子：
2.3
通讯作者：
Lee, Tack
中科院分区：
文献类型：
--
作者：
Jin, Long-Hu;Shin, Hwa-Yeon;Lee, Tack
目的：本研究的目的是研究大鼠化学性膀胱炎模型中膀胱炎症对膀胱功能的影响。我们还通过组织学证实了逼尿肌炎症对大鼠慢性膀胱炎症的影响。材料和方法：本研究共使用 13 只雌性 Sprague-Dawley 大鼠。 7只大鼠膀胱内灌注HCl诱发化学性膀胱炎，其余大鼠膀胱内灌注生理盐水作为假手术组。两周后，对代谢笼中所有未麻醉、不受约束的大鼠进行额外的腹内压测量，获得膀胱内压图。膀胱测压后立即处死大鼠。取出膀胱并进行组织学检查以了解肥大细胞和炎症变化。结果：与假手术大鼠相比，HCl 急性损伤大鼠的压力参数（包括基础压、阈压和最大膀胱压）没有差异。与假手术组相比，他们的膀胱容量、排尿量、残余量和排尿间隔均显着增加。与假手术组相比，他们还表现出逼尿肌过度活动的频率增加。膀胱内总压超过2 cmH(2)O时,逼尿肌过度活动的比例为56.3%。 HCl急性损伤大鼠的组织学表现与化学性膀胱炎一致。结论：下尿路症状和盆腔疼痛的重叠模式是间质性膀胱炎患者常见的疾病特征。正如本研究中通过组织学和清醒膀胱测量检查所观察到的，间质性膀胱炎动物模型的情况相似。然而，间质性膀胱炎模型在充盈期显示逼尿肌过度活动，但膀胱容量和排尿间隔没有减少，这与膀胱过度活动症患者的特征不同。
PURPOSE: The aim of this study was to investigate the effect of urinary bladder inflammation on bladder function in a rat chemical cystitis model. We also histologically confirmed the effects of inflammation in the detrusor on chronically inflamed bladder in rats.MATERIALS AND METHODS: A total of 13 female Sprague-Dawley rats were used in this study. In seven rats, intravesical instillation of HCl induced chemical cystitis, and the other rats with intravesical instillation of saline were used as the sham. After 2 weeks, cystometrograms were obtained with additional intraabdominal pressure measurements in all unanesthetized, unrestrained rats in metabolic cages. The rats were killed just after cystometry. The bladders were removed and examined histologically for mast cells and inflammatory changes.RESULTS: The rats with acute injury by HCl showed no differences in pressure parameters, including basal pressure, threshold pressure, and maximum bladder pressure, compared with the sham rats. They showed significantly increased bladder capacity, micturition volume, residual volume, and micturition interval compared with the sham group. They also showed an increased frequency of detrusor overactivity compared with the sham group. The percent of detrusor overactivity was 56.3% among the total intravesical pressure rises above 2 cmH(2)O. The histological findings of the rats with acute injury by HCl were consistent with chemical cystitis.CONCLUSIONS: Overlapping patterns of lower urinary tract symptoms and pelvic pain are common disease characteristics among interstitial cystitis patients. The situation in an animal model of interstitial cystitis is similar, as observed in this study by the histologic and awake cystometric examinations. However, the interstitial cystitis model showed detrusor overactivity during the filling phase without a decrease in bladder capacity and micturition intervals, which differs from the characteristics of overactive bladder patients.