CELL-PROLIFERATION IN NORMAL EPIDERMIS
CELL-PROLIFERATION IN NORMAL EPIDERMIS
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DOI：
10.1111/1523-1747.ep12261462
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发表时间：
1984-01-01
影响因子：
6.5
通讯作者：
ROSS, P
中科院分区：
文献类型：
--
作者：
WEINSTEIN, GD;MCCULLOUGH, JL;ROSS, P
在正常人表皮细胞增殖动力学的详细检查。使用[3 H]胸苷与放射自显影技术，增殖和分化的细胞动力学的定义和相互关联。正常表皮的增殖区室的细胞周期持续时间（Tc）为311小时，来自3个组成部分：发芽标记指数（LI），DNA合成的持续时间（TS），和生长分数（GF）。发芽指数为2.7% ±。1.2 Ts为14 h，后者由11例正常人的标记有丝分裂曲线的复合分数求得。从文献和从人皮肤异种移植到裸鼠中获得的GF为60%。银屑病患者的正常表皮似乎具有更高的增殖率。平均LI为4.2% ±。0.9，约比正常表皮高50%。然而，由于缺乏关于ts和GF的其他信息，还不能计算该组织的绝对细胞动力学值。描述了正常表皮中表皮细胞更新的动力学模型，其将角质形成细胞在3个表皮隔室中的出生/进入、运输和/或损失的速率相互关联：增殖的、有活力的分化的（马尔皮基层）和角质层。表皮中的预期动力学稳态通过每个隔室中非常相似的周转率（分别为1246、1417和1490个细胞/天mm 2表面积）证实。整个组织的平均表皮更新时间为39天。正常细胞的Tc为311 h，是银屑病Tc为36 h的8倍，是了解银屑病过度增殖病理生理过程所必需的。
A detailed examination of cell proliferation kinetics in normal human epidermis is presented. Using [3H] thymidine with autoradiographic techniques, proliferative and differentiated cell kinetics are defined and interrelated. The proliferative compartment of normal epidermis has a cell cycle duration (Tc) of 311 h derived from 3 components: the germinative labeling index (LI), the duration of DNA synthesis (ts), and the growth fraction (GF). The germinative LI is 2.7% .+-. 1.2 and ts is 14 h, the latter obtained from a composite fraction of labeled mitoses curve obtained from 11 normal subjects. The GF obtained from the literature and from human skin xenografts to nude mice is 60%. Normal-appearing epidermis from patients with psoriasis appears to have a higher proliferation rate. The mean LI is 4.2% .+-. 0.9, .apprx. 50% greater than in normal epidermis. Absolute cell kinetic values for this tissue, however, cannot yet be calculated for lack of other information on ts and GF. A kinetic model for epidermal cell renewal in normal epidermis is described that interrelates the rate of birth/entry, transit, and/or loss of keratinocytes in the 3 epidermal compartments: proliferative, viable differentiated (stratum malpighii), and stratum corneum. Expected kinetic homeostasis in the epidermis is confirmed by the very similar turnover rates in each of the compartments that are, respectively, 1246, 1417, and 1490 cells/day mm2 surface area. The mean epidermal turnover time of the entire tissue is 39 days. The Tc of 311 h in normal cells is 8-fold longer than the psoriatic Tc of 36 h and is necessary for understanding the hyperproliferative pathophysiologic process in psoriasis.