Microtubule-associated protein 2 (MAP2) -: a promising approach to diagnosis of forensic types of hypoxia-ischemia
Microtubule-associated protein 2 (MAP2) -: a promising approach to diagnosis of forensic types of hypoxia-ischemia
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DOI：
10.1007/s00401-005-1090-9
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发表时间：
2005-12-01
影响因子：
12.7
通讯作者：
Oehmichen, M
中科院分区：
文献类型：
--
作者：
Kühn, J;Meissner, C;Oehmichen, M
细胞骨架微管相关蛋白2（MAP 2）的神经元免疫反应性的丧失被认为是缺血后神经元至少短暂功能衰竭的标志。由于法医学类型的急性缺氧缺血性疾病没有特异性的神经病理学发现，因此检测这种相关的死亡原因往往是复杂的，可靠的缺血性生物标志物将非常重要。因此，我们调查了神经元的MAP 2的免疫反应性在人类案件的法医学意义。将对照组（n=27）与缺氧缺血组（n=45）进行比较，缺氧缺血组包括因上吊（n=19）、溺水（n=14）和一氧化碳（CO）中毒（n=12）而死亡。使用免疫组织化学染色，评估和比较海马（CA 1-CA 4区）和额叶皮质（II-VI层）中MAP 2阳性神经元的百分比。与对照组相比，缺氧缺血组海马CA 2-CA 4区和皮质II-VI层的MAP 2免疫染色均减少（P <0.05）。最脆弱的地区似乎是海马CA 4区和皮质层III-V。在缺氧缺血组，由于CO中毒死亡的特点是最低的MAP 2免疫反应。缺氧缺血组与对照组的不同之处在于MAP 2免疫染色明显减少。因此，MAP 2免疫反应性的损失可能支持法医类型的缺氧缺血性神经元损伤的诊断，虽然死后组织的调查必须谨慎解释。
The loss of neuronal immunoreactivity of the cytoskeletal microtubule-associated protein 2 (MAP2) is known to be a marker of-at least-transient functional failure of neurons following ischemia. Because there are no specific neuropathological findings in forensic types of acute hypoxia-ischemia, detection of this relevant cause of death is often complicated and a reliable ischemic biomarker would be of great importance. We therefore investigated the neuronal immunoreactivity of MAP2 in human cases of forensic significance. A control group (n=27) was compared to a group of cases of hypoxia-ischemia (n=45), comprising death due to hanging (n=19), drowning (n=14) and carbon monoxide (CO) poisoning (n=12). Using immunohistochemical staining, the percentage of MAP2-positive neurons in the hippocampus (areas CA1-CA4) and frontal cortex (layers II-VI) was evaluated and compared. The hypoxia-ischemia group showed decreased MAP2 immunostaining in the hippocampal areas CA2-CA4 (P < 0.05) and in cortical layers II-VI (P < 0.001) compared to controls. Most vulnerable regions seem to be the hippocampal CA4 area and cortical layers III-V. Within the hypoxia-ischemia group, death due to CO poisoning was characterized by the lowest MAP2 immunoreactivity. The hypoxic-ischemic groups differ from controls by a distinct decrease of MAP2 immunostaining. Thus, the loss of MAP2 immunoreactivity may support the diagnosis of neuronal injury in forensic types of hypoxia-ischemia, although investigations on postmortem tissue must be interpreted cautiously.