A human RNA polymerase II complex containing factors that modify chromatin structure
A human RNA polymerase II complex containing factors that modify chromatin structure
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DOI：
10.1128/mcb.18.9.5355
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发表时间：
1998-09-01
影响因子：
5.3
通讯作者：
Reinberg, D
中科院分区：
文献类型：
--
作者：
Cho, H;Orphanides, G;Reinberg, D
我们分离出一种人类 RNA 聚合酶 II 复合物，其含有染色质结构重塑活性和组蛋白乙酰转移酶活性。除了 RNA 聚合酶 II 之外，该复合物还包含 Srb 蛋白、Swi-Snf 复合物以及组蛋白乙酰转移酶 CBP 和 PCAF。值得注意的是，该复合物中不存在一般转录因子。该复合物通过两种不同的方法纯化：常规色谱法和使用针对 CDK8（酵母 Srb10 蛋白的人类同源物）的抗体的亲和色谱法。蛋白质相互作用研究表明 RNA 聚合酶 II 与组蛋白乙酰转移酶 p300 和 PCAF 之间存在直接相互作用。重要的是，p300 与非磷酸化、具有起始能力的 RNA 聚合酶 II 发生特异性相互作用。相反，PCAF 与具有延伸能力的磷酸化形式的 RNA 聚合酶 II 相互作用。
We have isolated a human RNA polymerase II complex that contains chromatin structure remodeling activity and histone acetyltransferase activity. This complex contains the Srb proteins, the Swi-Snf complex, and the histone acetyltransferases CBP and PCAF in addition to RNA polymerase II. Notably, the general transcription factors are absent from this complex. The complex was purified by two different methods: conventional chromatography and affinity chromatography using antibodies directed against CDK8, the human homolog of the yeast Srb10 protein. Protein interaction studies demonstrate a direct interaction between RNA polymerase II and the histone acetyltransferases p300 and PCAF. Importantly, p300 interacts specifically with the nonphosphorylated, initiation-competent form of RNA polymerase II. In contrast, PCAF interacts with the elongation-competent, phosphorylated form of RNA polymerase II.