PROLACTIN RECEPTOR IS ASSOCIATED WITH C-SRC KINASE IN RAT-LIVER
PROLACTIN RECEPTOR IS ASSOCIATED WITH C-SRC KINASE IN RAT-LIVER
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DOI：
10.1210/me.9.11.1461
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发表时间：
1995-11-01
影响因子：
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通讯作者：
GARCIARUIZ, JP
中科院分区：
文献类型：
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作者：
BERLANGA, JJ;VARA, JAF;GARCIARUIZ, JP
在哺乳大鼠肝细胞中研究了垂体激素PRL的作用机制。从肝裂解物获得的PRL受体免疫复合物具有相关的酪氨酸激酶活性。酪氨酸激酶在分离的肝细胞中被鉴定为pp 60（c-src）。用PRL孵育肝细胞诱导PRL受体与pp 60（c-src）的缔合以及由此产生的对其酪氨酸激酶活性的刺激。此外，PRL刺激c-fos、c-jun和c-src的基因表达。所有这些发现支持pp 60（c-src）酪氨酸激酶参与促进肝细胞中细胞生长的PRL细胞内信号传导的早期步骤的想法。
The mechanism of action of the pituitary hormone PRL was studied in hepatocytes of lactating rats. PRL receptor immune complexes obtained from liver lysates have an associated tyrosine kinase activity. The tyrosine kinase has been identified in isolated hepatocytes as pp60(c-src). Incubation of hepatocytes with PRL induces the association of PRL receptor with pp60(c-src) and the resultant stimulation of its tyrosine kinase activity. Furthermore, PRL stimulates the gene expression of c-fos, c-jun, and c-src. All of these findings support the idea that the pp60(c-src) tyrosine kinase participates in the early steps of the PRL intracellular signaling that promotes cell growth in liver cells.