Drug-Loaded Nanoparticles from 'Ershiwuwei Shanhu' Pill Induced Cellular Swelling of SH-SY5Y Neuroblastoma Cells
Drug-Loaded Nanoparticles from 'Ershiwuwei Shanhu' Pill Induced Cellular Swelling of SH-SY5Y Neuroblastoma Cells
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“二十五味珊瑚”丸载药纳米颗粒诱导 SH-SY5Y 神经母细胞瘤细胞肿胀
DOI：
10.1166/jnn.2016.10944
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发表时间：
2016-03-01
影响因子：
--
通讯作者：
Qiu, Xiaozhong
中科院分区：
文献类型：
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作者：
Liu, Yali;Song, Xiaoping;Qiu, Xiaozhong
Drug-loaded nanoparticles from 'Ershiwuwei Shanhu' Pill (ESP) inducing cellular swelling of the SH-SY5Y neuroblastoma cells were investigated. Electron microscope was used to observe nanoparticles existing in the freeze-dried supernatant of 'Ershiwuwei Shanhu' Pill. Drug-free nanoparticles were obtained from the solution of drug-loaded nanoparticles via dialysis. The size and zeta potential of two kinds of nanoparticles were tested by granularmetric analysis and surface charge analysis. Results showed that nanoparticles could penetrate into cellular nucleus and caused cell swelling. CCK8 analysis implied that low concentration of drug-free nanoparticles from 'Ershiwuwei Shanhu' Pill can induce cell proliferation of the SH-SY5Y neuroblastoma cells, while drug-loaded nanoparticles can reduce cell viability through NF-kappa B pathway. Drug-loaded nanoparticles existed in 'Ershiwuwei Shanhu' pill might play a vital role during pharmacotherapy, which served as nanocarriers in delivering drugs into cells.
