Retinal synaptic regeneration via microfluidic guiding channels.
Retinal synaptic regeneration via microfluidic guiding channels.
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DOI：
10.1038/srep13591
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发表时间：
2015-08-28
影响因子：
4.6
通讯作者：
Qin L
中科院分区：
文献类型：
--
作者：
Su PJ;Liu Z;Zhang K;Han X;Saito Y;Xia X;Yokoi K;Shen H;Qin L
体外培养游离视网膜神经元是研究视网膜突触再生（RSR）和探索人工视网膜电位的重要模型。为了重建视网膜神经元突触，我们将视网膜前体细胞培养在具有多个微通道阵列的微流体芯片中。培养的视网膜细胞通过微通道物理连接。通过甘氨酸能因子的施用，细胞通过微通道激活电信号转导。此外，采用基于图像的分析方法来量化突触连接并评估突触再生动力学。在抑制剂甘氨酸浓度低于100 μM时，RSR率显著下降，随后在较高浓度下趋于相对稳定。此外，氯化钾化学刺激可提高RSR。总之，微流控突触再生芯片为在细胞水平上高通量研究RSR提供了一种新的工具，并可用于视网膜前体细胞移植的质量控制。
In vitro culture of dissociated retinal neurons is an important model for investigating retinal synaptic regeneration (RSR) and exploring potentials in artificial retina. Here, retinal precursor cells were cultured in a microfluidic chip with multiple arrays of microchannels in order to reconstruct the retinal neuronal synapse. The cultured retinal cells were physically connected through microchannels. Activation of electric signal transduction by the cells through the microchannels was demonstrated by administration of glycinergic factors. In addition, an image-based analytical method was used to quantify the synaptic connections and to assess the kinetics of synaptic regeneration. The rate of RSR decreased significantly below 100 μM of inhibitor glycine and then approached to a relatively constant level at higher concentrations. Furthermore, RSR was enhanced by chemical stimulation with potassium chloride. Collectively, the microfluidic synaptic regeneration chip provides a novel tool for high-throughput investigation of RSR at the cellular level and may be useful in quality control of retinal precursor cell transplantation.