Gonadotropin-induced steroidogenic shift towards maturation-inducing hormone in Japanese yellowtail during final oocyte maturation
Gonadotropin-induced steroidogenic shift towards maturation-inducing hormone in Japanese yellowtail during final oocyte maturation
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日本黄尾鱼卵母细胞最终成熟过程中促性腺激素诱导的类固醇生成向成熟诱导激素的转变
DOI：
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
M. Matsuyama
中科院分区：
文献类型：
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作者：
M. Rahman;K. Ohta;H. Chuda;S. Nakano;Keigo Maruyama;M. Matsuyama
从未处理和人绒毛膜促性腺激素（HCG）处理的日本黄尾五点红尾鱼（Seriola quinqueradiata）的不同成熟阶段获得的完整卵泡，与放射性[ 3 H]双烯醇酮、[ 3 H]17-羟孕酮或[ 14 C]雄烯二酮以及经薄层色谱（TLC）鉴定的类固醇代谢物孵育，然后重结晶至恒定比活度。在未处理的卵黄发生晚期（0 h）卵泡中，雄烯二酮是主要产物，睾酮和雌二醇-17 β含量较少。在卵黄形成后（注射后12 h）的完整卵泡中，雄烯二酮占主导地位，尽管不产生睾酮和雌二醇-17 β，但有少量的17，20 β-二羟基-4-异戊烯-3-酮（17，20 β-P）和17，21-二羟基-4-异戊烯-3，20-二酮（11-脱氧皮质醇）。在HCG处理的鱼中，类固醇生成的转变导致睾酮和雌二醇-17 β的消失与17，20 β-P的出现相一致。在早期和晚期最终卵母细胞成熟FOM期间（注射后24和36 h），17，20 β-P的产生增加了5至7倍，而11-脱氧皮质醇的产生几乎保持不变。在FOM期间，除了17，20 β-P外，还合成了其5β-还原代谢产物17，20 β-二羟基-5 β-吡喃-3-酮（5β-17，20 β-P），表明诱导成熟的17，20 β-P活性降低。17，20 β，21-三羟基-4-异戊烯-3-酮（20β-S）在日本黄尾鱼任何成熟阶段的卵巢碎片中都不合成。对FOM期间在TLC上鉴定的代谢物进行了检测，以在体外生物测定中评价其成熟诱导活性。17，20 β-P是最有效的生殖囊泡破裂（GVBD）诱导剂，其次是5β-17，20 β-P。17，20 β-P在FOM前和FOM期间的及时合成以及其在体外诱导GVBD的巨大效力支持了17，20 β-P作为日本黄尾鱼成熟诱导激素的生理作用的证据。
Intact ovarian follicles, obtained from untreated and human chorionic gonadotropin (HCG) treated Japanese yellowtail Seriola quinqueradiata during different maturational stages, were incubated with radioactive [ 3 H]pregnenolone, [ 3 H]17-hydroxyprogesterone or [ 14 C] androstenedione and steroid metabolites identified by thin layer chromatography (TLC) followed by recrystallization to constant specific activity. In untreated late vitellogenic (0 h) follicles, androstenedione was the major product with smaller amounts of testosterone and oestradiol-17β. In post-vitellogenic (12 h post-injection) intact follicles, androstenedione predominated, and although testosterone and oestradiol-17β were not produced, there were small amounts of 17,20β-dihydroxy-4-pregnen-3-one (17,20β-P) and 17,21-dihydroxy-4-pregnene-3,20-dione (11-deoxycortisol). In HCG-treated fish, a steroidogenic shift resulted in the disappearance of testosterone and oestradiol-17β coinciding with the appearance of 17,20β-P. During early and late final oocyte maturation FOM (24 and 36 h post-injection), there was a five- to seven-fold increase in the production of 17,20β-P, whereas production of 11-deoxycortisol remained almost the same. During FOM, in addition to 17,20β-P, its 5β-reduced metabolite, 17,20β-dihydroxy-5β-pregnan-3-one (5β-17,20β-P) was synthesized, suggesting a decrease in maturation-inducing 17,20β-P activity. 17,20β,21-Trihydroxy-4-pregnen-3-one (20β-S) was not synthesized by ovarian fragments in Japanese yellowtail at any maturational stage. The metabolites identified on TLC during FOM were tested to evaluate their maturation-inducing activity in an in vitro bioassay. Of the steroids tested, 17,20β-P was the most effective inducer of germinal vesicle breakdown (GVBD), followed by 5β-17,20β-P. Timely synthesis of 17,20β-P immediately prior to and during FOM as well as its great potency in inducing GVBD in vitro supports the evidence for a physiological role of 17,20β-P as a maturation-inducing hormone in Japanese yellowtail.
类固醇在体外诱导大西洋黄花鱼卵母细胞生发囊泡破裂中的构效关系。
影响因子：
2.7
作者：
Trant,JM;Thomas,P
通讯作者：
Thomas,P
影响因子：
3.6
作者：
King, W;Ghosh, S;Sullivan, CV
通讯作者：
Sullivan, CV